Inhibitive effects of genistein and quercetin on NIH3T3 fibroblast proliferation and collagen synthesis / 第二军医大学学报

Objective:To study the effects of genistein (GE) and quercetin (QU) on proliferation and collagen synthesis of mouse fibroblast NIH3T3 cells.Methods:Cell proliferation was measured by crystal violet staining assay.Collagen synthesis was determined by 3H proline incorporation assay.Results:GE (25 70 ?mol?L -1 ) and QU (6.25 50 ?mol?L -1 ) concentration dependently reduced the serum driven increase of cell proliferation and collagen synthesis.GE and QU also attenuated platelet derived growth factor (PDGF) driven proliferative activity and transforming growth factor ?1 (TGF ?1) driven collagen synthesis.Conclusion:GE and QU can inhibit mouse fibroblast NIH3T3 cell proliferation and collagen synthesis in vitro .