Impact of pedicle screw placement techniques on the cranial facet joint violation and related risk factors / 中华骨科杂志

Facet joint is the important part of spinal biomechanical structures. The damage of facet joint will destroy the stability of spinal motion segments and accelerate the adjacent segments degeneration (ASD). The violation of cranial facet joint based on various screw insertion techniques is a common but easily overlooked factor in clinical application. The reduction of intra-operative cranial facet joint violation is essential for reduction of postoperative ASD. The rates of facet violation are related to screw insertion techniques. The insertion techniques, including robot-assisted guidance, computer-assistant navigation, and cortical bone trajectory, are used successfully well in protecting cranial facet joints, compared with the freehand pedicle screw placement technique, which has been widely utilized in clinical practice. However, the conventional X-ray guided screw insertion technique is associated with a higher rate of facet violation. The main reasons why different techniques lead to various rates of facet violation include various anatomical referenced landmarks during screw insertion, selection of assisted equipment for instruments, and resistance of soft tissues of the spine. In addition, the related risk factors, such as facet angle, screw insertion segments, lumbar degeneration, and learning curve effect, can also affect the rates of facet violation. In the present study, we compared the differences of facet joint violation when using various screw techniques, and summarized the causes of violations and related risk factor. The present review might provide references for surgeons regarding the decrease of cranial facet joint violation, optimization of insertion techniques and reduction of ASD.

Effects of Yiqi Jianpi decoction on inflammatory reaction, immune function and nutritional status of patients after laparoscopic rectal cancer surgery / 中国中西医结合急救杂志

Objective To explore the effects of Yiqi Jianpi decoction on inflammatory reaction, immune function and nutritional status in patients after surgery of laparoscopic rectal cancer. Methods Eighty-two patients with post-operative laparoscopic rectal cancer resection admitted to Pingxiang People's Hospital from June 2017 to March 2019 were enrolled, and according to whether traditional Chinese medicine (TCM) was used or not, they were divided into a western medicine treatment control group (control group) and a combined traditional Chinese and western medicine treatment group (combined group), 41 cases in each group. The control group was treated with symptomatic conventional western therapy, while the combined group was additionally treated with Yiqi Jianpi decoction based on the conventional western treatment as in the control group; the composition of the decoction: hedyotis herba, herba agrimoniae, radix astragali each 30 g; radix codonopsis, ganoderma lucidum each 20 g; rizoma atractylodes, poria cocos and prunella chinensis 15 g each; pinellia ternata 9 g, licorice root 6 g; all the above ingredients were together boiled with water and the fluid (decoction) was filtered, 150 mL once orally taken, twice a day, 21 days constituting one therapeutic course, and the clinical efficacy was evaluated after 1 course of therapy. The changes of TCM syndrome score, simple health situation scale (SF-36) score, inflammatory indexes, immune function and nutritional status of the two groups were observed before and after treatment. Results After treatment, the levels of TCM syndrome score, C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and CD8+ in the two groups were significantly lower than those before treatment, while the levels of SF-36 score, CD4+, CD4+/CD8+, transferrin (TRF) and albumin (Alb) were significantly higher than those before treatment (all P < 0.05), and the degree of changes in the combined group were more significant than those in control group [TCM syndrome score: 3.79±2.22 vs. 6.86±2.02, SF-36 score:75.18±4.13 vs. 64.17±4.01, CRP (mg/L): 4.54±1.31 vs. 5.71±2.21, IL-6 (μg/L): 20.21±2.21 vs. 26.12±2.13, TNF-α (μg/L): 33.04±4.56 vs. 36.41±3.23, CD4+: 0.37±0.03 vs. 0.35±0.03, CD8+: 0.25±0.03 vs. 0.28±0.03, CD4+/CD8+: 1.51±0.39 vs. 1.19±0.37, TRF (g/L): 3.05±0.41 vs. 2.28±0.42, Alb (g/L): 43.88±2.28 vs. 39.86±2.03, all P < 0.05]. Conclusion Yiqi Jianpi decoction in the treatment of patients after surgery of laparoscopic rectal cancer can effectively promote the recovery of gastrointestinal function, reduce the inflammatory reaction, elevate the immune function and improve clinical prognosis.

Effects of short-chain fatty acids on oxidative stress and inflammation in glomerular mesangial cells induced by high glucose / 中华内分泌代谢杂志

To investigate the effects of short-chain fatty acids(SCFAs)on oxidative stress and inflammation in cultured glomerular mesangial cells(GMCs)under high glucose.SV-40 MES 13 mouse GMCs were exposed to 30 mmol/L glucose, exogenous SCFAs or their specific G-protein coupled receptor 43(GPR43)agonist were used individually as an intervention.GPR43 expression was suppressed by transfection with GPR43-specifc siRNA.The oxidative stress-related factors reactive oxygen species and malondialdehyde were detected and the expression of GPR43,monocyte chemotactic protein 1(MCP-1),and interleukin-1β(IL-1β)were observed.GPR43 expression were significantly down-regulated,but reactive oxygen species and malondialdehyde production and MCP-1 and IL-1β releases were induced by high glucose(all P<0.05),treatment with SCFAs or GPR43 agonist reversed high glucose-induced oxidative stress and inflammation in a dose-dependent manner(all P<0.05).However, these SCFAs-mediated effects were blunted by siRNA-mediated knockdown GPR43(all P<0.05).SCFAs mitigates high glucose-induced oxidative stress and inflammatory injury in part via GPR43 signaling pathway,suggesting a likely mechanism for SCFAs-induced therapeutic effect in diabetic kidney disease.

Role of sweet taste receptors in the activation of NLRP3 inflammasome signaling in diabetic kidney disease / 中华内分泌代谢杂志

Objective@#To investigate the role of sweet taste receptors(STRs)in the activation of reactive oxygen species (ROS)-NLRP3 inflammasome signaling in diabetic kidney disease(DKD).@*Methods@#DKD mouse were established by high-fat diet and streptozotocin. After mouse glomerular mesangial cells(GMCs)were exposed to high glucose, STRs(T1R2/T1R3)and associated signaling components and NLRP3 inflammasome signaling components expressions were detected. After GMCs were treated with STRs inhibitor lactisole, the production of ROS was detected and the role of STRs in the activation of NLRP3 signaling was investigated.@*Results@#Under high glucose condition, the expressions of T1R2, T1R3, Gα-gustducin, phospholipase C-β2, and TRPM5 were significantly decreased in vivo and in vitro(all P<0.05)and ROS-NLRP3 signaling was activated(all P<0.05). Lactisole significantly mitigated the production of ROS and the activation of NLRP3 inflammasome signaling stimulated by high glucose in GMCs(all P<0.05).@*Conclusion@#The STRs(T1R2/T1R3)-mediated signaling pathway may be involved in the regulation of ROS-NLRP3 inflammatory signaling, suggesting that STRs may act as new therapeutic targets of DKD. (Chin J Endocrinol Metab, 2018, 34: 587-593)

Expression and spatial distribution of P2X7 receptor in pilocarpine-induced epileptic rat hippocampus / 中南大学学报(医学版)

Objective:To investigate the dynamic expression and spatial distribution of P2X7 receptor in pilocarpine-induced epileptic rat hippocampus.Methods:Status epilepticus (SE) model of rats was established by intraperitoneal injection with chloride lithium and pilocarpine.Rat brain tissue and hippocampus were collected at 1,3,7,14,28 days after SE.The protein expression of P2X7 receptor in rat hippocampus was detected by Western blot.The distribution of P2X7 receptor in hippocampal sub-region was analyzed by immunohistochemistry.Results:Bilateral forelimb clonus appeared at (33.9±12.3 min after intraperitoneal injection with pilocarpine.The protein expression of P2X7 receptor was increased at 1d after SE,while it was decreased gradually from 3 d to minimum at 7 d,then it was elevated continuously to 28 d.Among them,the expression of P2X7 receptor was increased significantly at 1,14 and 28 d post-SE (P＜0.05).Immunohistochemical staining showed that P2X7 receptor was detected in all areas.The expression pattern of P2X7 receptor in hippocampal DG and CA3 area was consistent with protein expression,but its expression in hippocampal CA1 area was not significantly changed after SE.Conclusion:The expression of P2X7 receptor in post-SE hippocampus is in a time-dependent manner and spatial specificity.P2X7 receptor might be involved in the development of chronic epilepsy.

Effect of trait anxiety and gender difference on risk-avoidant in decision-making in 316 college students / 中国心理卫生杂志

Objective: To explore the relationship of trait anxiety, gender difference and risk averse. Methods: A test was conducted fwstly in 328 college students with the State-Trait Anxiety Inventory. The participants with a score≥53 were termed as high trait-anxiety group (N=30), in contrast to the former, the par-ticipants with a score ≤ 30 were treated as the low trait-anxiety (N=30) . The material was 10 hypothesized risk settings, asking the participants to do decision-making, at the same time, the participants were also required to ac-cess their subjective cost if the result of their decision was negative; recording the scores and the reaction time of the participants. Results: The risk-avoidant score in high trait-anxiety group was significantly higher than that in low trait-anxiety group [(5.97±1.53) vs.(3.27±1.25), P <0.01], and the risk-avoidant score in males was sig-nificantly higher than that in females [(2.78±1.37) vs.(6.46±1.26), P <0.01] .There also had significant interaction effects between the 2 variables (F=7.43, P <0.01). The reaction time in males was also longer than that in females [high-trait anxiety: (1586.47±10.352)vs.(1067.52±18.468); low-trait anxiety: (643.46±36.759)vs.(498.36±9.673); P < 0.01] . The correlation between subjective loss and trait-anxiety in females was higher than that in males (Z=2.15, P<0.05) . Conclusion: There is significant effect of trait-anxiety and gender difference on risk-avoidant decision-making. The high-trait and female individuals have more risk-avoidant in decision-making.