[Erythrocyte Parameters changes through out Pregnancy in women Referred to gynecology and obstetrics clinic at Khanevadeh medical center at 2004-2005 year]

Background: the erythrocyte parameters are subjects to change through out pregnancy. To differentiate the aforementiond chages with the iron defiency anemia, one is obliged to perform serial blood sampling and erythrocyte parameters tests. The current study was designed to monitor erythrocyte parameter changes during pregnancy

Materials and methods: 350 patients were recruited to a descriptive observational cross sectional study through out their Pregnancy. General data including maternal age,pregnancy age in accordance with the lmp, times of gravidity and delivery, iron and folic acid complementation were assessed blood sampling and erythrocyte parameters. Assessment were used to qualify the gaind data's meaningfulness. The pvalue was considered equal with 0.05

Results: 308 pateints results were finalized. The mean age was 27.04 +/- 4 years. Most pregnancy pregnancy cases wrere in the first time pregnancy subgroup [77.85%]. Most delivery cases were nuliparous subgroup [74.16%]. The iron and folic acid supplementation were dominant in 95.13% of cases, respectively 2.68% of attendants were smokers, hemogtobin, hematocrite and mchc were decreased with pregnancy progress. In the mean while mcv increases through out pregnancy Mch shows no meaningful changes

Conclusions: the values of hemoglobin and hematocrite decrease through out pregnancy And macrocytic changes occur through out a physiologic and normal pregnancy

[Preparation of neuraminidase specific antiserum from H9N2 subtype of avian influenza virus]

In this research, affinity chromatography have been developed and standardized for production of Neuraminidase antigen of influenza virus for preparation of monospecific antiserum in rabbits. Avian influenza Virus stocks [A/chicken/Iran/259/1998/[H9N2]] were propagated in the allantoic cavities of 10-day old embryonated chicken eggs. The harvested suspension was concentrated by polyethylenglycol 6000. Concentrated samples were layered onto sucrose gradient [30-60%]. Both hemagglutinin and neuraminidase were solubilized from purified viruses with Triton X-100, across 30% sucrose gradient. NA was isolated from HA and other viral proteins by affinity chromatography on N- [paminophenyl] oxamic acid. Fractions that had high NA-activity and did not show HA activity were pooled and analyzed by neuraminidase inhibition and SDS-PAGE. For preparation of antisera, rabbits were immunized by purified NA and Freund's adjuvant at three weeks interval, and sera collected 7 days after boosting. In SDS-PAGE no viral protein band detected except for single band in the position of NA. NA activity of purified protein was 3.8 x 104 NA units. Enzymatic activity of Neuraminidase purified by this procedure decrease sharply above 48°C. The purified neuraminidase was producing a significant antibody response in agar gel precipitation. No reaction was observed with neuraminidase specific antiserum and H9-HA of the same virus. According to virtual purity and enzymatic activity of purified neuraminidase and highest avidity and specificity of antiserum, it was speculated that optimized protocol can be directly applied to produce antigen and antiserum from all subtypes of virus and can be easily used in commercial diagnostic tests