[Minimum inhibitory concentration of silver nanoparticle against Staphylococcus aureus and its relation with Methicillin resistance and bacterial source of isolation]

Background and Objective: The rise of antibiotic resistance particulary Methicillin resistance in pathogenic bacteria such as Staphylococcus aureus is found to be an emerging threat to human health especially in hospitals. Heavy metal nanoparticles such as Ag used for inhibition of this bacterium. This study was done to determine of minimum inhibitory concentration [MIC] Ag nanoparticle against Staphylococcus aureus which isolated in Gorgan, north of Iran and its relation with Methicillin resistance and source of bacteria

Methods: In this descriptive - analytical study, the MIC Ag nanoparticle in 183 isolates of Staphylococcus aureus by microdilution method was determined. 30 isolates, based on mecA gene was considered as MRSA. Samples were collected from patients, nose of healthy carriers and foods. Compare the MIC of isolates based on Methicillin resistance, source of the bacteria and resistance to other antibiotics were assessed

Results: Out of 183 samples MIC was varied from 1 to 16 micro g/ml, and mean +/- std was 2.9 +/- 1.89 micro g/ml. MIC mean of silver nanoparticles in isolated from foods were 2 +/- 0.7, isolared from healthy carriers were 4.1 +/- 2.4 and from patients were 3.4 +/- 2.1 micro g/ml and were statically significant [P<0.05]. MIC mean of silver nanoparticles in MSSA isolates are 3.9 +/- 2.3 and in MRSA isolates are 2.4 +/- 1.4 micro g/ml that were statically significant [P<0.05]. MIC mean of gentamycin resistant isolate were lower than sensitive one. But between MIC of silver nanoparticles and other antibiotics resistance was not significant statistically

Conclusion: There is a relation between silver nanoparticle MIC, source of sample isolation, Methicillin and gentamycin resistance. Since MIC of silver nanoparticles on isolates of Methicillin resistant is low, the possibility of its use in the control of MRSA in hospital infections can be considered as a prime attention the Gentamycine

[Is Epstein-Barr virus associated with pterygium?]

Pterygium is a fibrovascular lesion of the ocular surface with unknown origin, decrease in the vision. This study was done to evaluate the possible role of Epstein-Barr virus [EBV] in the formation of pterygia. This case-control study was done on 50 tissue specimens of pterygium from the patients who underwent pterygium surgery as the case group and 10 conjunctival biopsy specimens of individuals without pterygium including the patients whom underwent cataract surgery, as controls. The evidence of EBV infection was tested by polymerase chain reaction [PCR]. EBV was detected in three [6%] patients with pterygia. EBV was not detected in controls. There was not any significant correlation between pterygium and the presence of EBV. According to this study, EBV virus is not associated with pterygium formation

[Association between anti- beta 2GP1 autoantibody with Acute Coronary Syndrome]

Atherosclerosis is a chronic autoimmune inflammatory disease that remains the most common cause of death in the globe. This affects the vascular wall and leads to coronary artery diseases. Several studies have evaluated the association between auto-antibodies and cardiovascular disease. One of the candidate autoantigens is the Beta 2-glycoprotein I [beta 2GPI]. Determining risk factor of the coronary heart diseases is of huge significance, so we carried out the comparative study of Anti- beta 2GP1 autoantibody level in patients with Acute Coronary Syndrome and healthy controls in Gorgan, Iran. A case-control, study was conducted on 179 subjects in 2007 in whom 79 subjects were healthy [Control group] and 100 subjects had coronary artery disease [Case group]. A questionnaire was filled by all subjects. Blood and serum samples were assessed by commercial ELISA tests measuring IgM and IgG auto-antibodies to beta 2GP1 antigen. Data were analyzed using student t-test and Fisher test. The positivity of IgG and IgM isotypes for anti- beta 2-GPI antibodies in serum of the cases were%68.6 and%43, respectively. Our results also indicated that frequency of IgG and IgM autoanti-beta2-GPI isotypes in serum of the controls were%48.1 and%2.5, respectively. The results from the serum of the subjects showed that IgG-antibody against beta 2GPI was found in%50 of the female cases and%73.2 of the male cases [P<0.01]. On the other hand, this values were%48.5 of females and%47.8 of males in control group [P<0.05]. Our finding shows that there is a relation between serum levels of IgG-antibodies against beta 2GP and coronary artery diseases