Isolation, molecular identification and evaluation of antifungal effects of the bacilli isolated from rhizospheric soil of gorgan region against tricopfifton mentegrqp aytes

Soil bacteria, particularly Bacillus genus have the potential of producing a range of bioactive substances with antimicrobial and antifungal properties. They have the ability to produce hundreds of active and effective biologic compound against microorganisms. Therefore, it seems to be a proper candidate in the biocontrol of fungal pathogenesis. In this study, soil samples were collected from different parts of Gorgan in order to isolate Bacillus and to determine their antifungal activity against T.mentagrophytes. The isolates that had the highest antifungal effects were analyzed by PCR and 16 srRNA sequencing. Of 54 strains, 14 have antifungal activity. The Isolates, S4 and SI2, identified as B.cereus and B.thuringiensis respectively show the highest antidermatofit effect. These isolates based on 16s rRNA sequence analysis show 97% honiology with Bacillus cereusstrain KU4 and Bacillus thurmgienslsstrain ucsc 27. According to the results, it seems that the soil Bacilli have biocontrol potential against dermatophytic agents such as T.mentagrophytes

[Specular microscopy evaluation of corneal endothelial cells after intravitreal and subconjuctival injection of avastin]

To evaluate corneal endothelial cell changes after intravitreal and subconjunctival injection of avastin [bevacizumab] based on specular microscopic findings. This prospective interventional study was performed on 82 eyes of 82 patients. Forty-two cases with ptrygia received a subconjunctival injection of bevacizumab [2.5mg/0.2ml] and 40 diabetic subjects with retinal neovascularization or macular edema received an intravitreal injection of bevacizumab [1.25mg/0.1ml]. Specular microscopy, including corneal endothelial cell count, size and shape, was performed in all patients for the injected eye before, and one and three month after injection and the results were compared and analyzed. There was no change in the corneal endothelial cells count after one month but there was mild reduction of endothelial cells after 3 months which was not statistically significant.[P=0.75 and 0.29, respectively]. Subconjunctival and intravitreal injection of bevacizumab [up to 1.25 mg/0.1 ml and 2.5 mg/0.2 ml, respectively] entails no harmful effect on human corneal endothelial cells up to three months following the injection

[Comparison of antimicrobial effect of Parstinaca sativa [Zardak] and Daucus carota on Listeria monocytogenes]

The antilisterial properties of some vegetables such as Daucus carota have already been reported, but there is no report on Zardak [Parstinaca sativa]. The objective of this study was comparison of the inhibitory effect of Zardak and Daucus carota [shredded and concentrations of juice] on two suspensions of Listeria [L.] monocytogenes. In this experimental study, contamination of two carrot cultivars was examined according to national standard no. 8035-1. Then carrot treatments prepared. Two suspensions [10[3] and 10[5] CFU/ml] of L. monocytogenes serotype 1295 PTCC inoculated on shredded and concentrations of carrot juices. After 1 and 24 h in refrigerator, inoculations were cultured on Listeria Selective Agar. The results reported according to the difference between the typical Listeria colonies on inoculated and control plates. Data were analyzed by Kruskal-Wallis test. P-Values lower than 0.05 was considered significant. Carrot samples had no Listeria contamination. Statistical analysis showed both cultivars had significant inhibitory effect on L. monocytogenes, but it is more in Zardak than Daucus carota [p<0.005]. The inhibitory effect of Zardak was shown on both bacterial concentrations, but Daucus carota effect only on 10[3] CFU/ml. The effect of Zardak became more after 24 h in refrigerator than 1 h [p<0.05], but it made no difference in Daucus carota. Whole carrot juice had more effect than other treatments [p<0.05]. We noticed fresh carrots had no inhibitory effect, but it could be seen by keeping carrots in refrigerated or room temperature for 4 days. Both Zardak and Daucus carota have antilisterial properties, but the effect of Zardak was mere significant. Variable inhibitory conditions would be objectives of other researches. On the other hand, probability of extracting antilisterial compounds from Zardak and using as a natural food preservative is suggested

First report of Pasteurella dagmatis isolation from a bitch urine in Iran

Pasteurella species are commensal bacteria of the respiratory airways and oronasal cavity of animals. In this study, we report an unusual case of Pasteurella dagmatis isolation from dog urine with uroliths. An 11-year-old female dog was admitted to the Veterinary Hospital of Ahvaz University complaining of abdominal pain, hematuria, dysuria and stranguria. In clinical and radiological examinations, it was found that abdomen was distended due to urinary bladder obstruction with uroliths. Pasteurella dagmatis was isolated from urine specimen collected by cystocentesis. Finally, uroliths were removed by surgery. Appropriate antibiotic treatment with cephalexin caused improvement. To the best of our knowledge, this is the first report of P. dagmatis presence in dog urine

[Preliminary study of potential toxic effects of taranjebin in Syrian mice]

Manna taranjebin is produced by an insect, Poophilus nebulosus, on camel's thorn plant. The manna has been traditionally used in herbal medicine and recent studies have studied/confirmed some of its therapeutic effects. In this study, some potential toxic effects of taranjebin were studied. In the first experiment, 4 groups of 6 mice [25.6 +/- 0.40g] received different doses of taranjebin [0.6-4.8g/kg/d] orally. The control group [n=6] received similar volumes [10ml/kg] of normal saline. In the second experiment, different groups of mice [n=5, 25.6 +/- 0.45g] were injected similar doses of taranjebin or placebo intraperitoneally. In both studies, the mice were watched for clinical sings during 10 days of experiments. Weight gains were measured in both experiments, and the mice in the second experiment were blood sampled for assessment of serum urea, creatinine, total bilirubin, alkaline phosphatase [ALP] and alanine aminotransferase [ALT]. No significant differences, regarding weight gain or the studied serum parameters, were observed between the control groups and those receiving taranjebin. It is concluded that even in high doses, taranjebin does not exert detectable toxic effects in the studied variables

Comparison of PCR and bacterial culture methods for diagnosis of dairy cattle's subclinical mastitis caused by staphylococcus aureus

To compare PCR and bacterial culture methods for diagnosis of subclinical mastitis caused by Staphylococcus aureus, 100 milk samples from cattle with subclinical mastitis and 20 samples from healthy cattle were collected and tested. The samples were cultured on selective blood agar and bacteria were identified by standard methods. DNA extracted from samples was subjected to PCR reaction with species specific primers and PCR products were analyzed by agarose gel electrophoresis. Based on the PCR results the prevalence of subclinical mastitis due to S. aureus was 25%. In the bacteriological culture of single milk sampling, S. aureus was isolated from the same samples being positive in PCR. A correlation of 100% was found between PCR and single milk sampling culture method by Mc Nemar test. All of the CMT negative samples were also negative in culture and PCR methods. The results of this study indicate that the PCR reaction is sensitive and specific for diagnosis of S. aureus in subclinical mastitis and can detect this pathogen in milk samples at species level in few hours