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EMHJ-Eastern Mediterranean Health Journal. 2001; 7 (4-5): 756-762
em Inglês | IMEMR | ID: emr-157992

RESUMO

The presence of genes for verotoxin 1 and 2 [VT1 and 2] among sorbitol non-fermenting Escherichia coli isolates from diarrhoeal cases was assessed using polymerase chain reaction assay. Of 60 [88%] positive isolates, 37 [62%] harboured VT1 and 23 [38%] both VT1 and VT2. In HeLa cell adherence assay, 48 [71%] isolates exhibited mannose-resistant adherence to HeLa cells. Multidrug resistance was observed in 56 [82%] isolates, with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim and tetracycline pattern being the most common. There were 13 common and 22 single biochemical phenotypes identified. Isolates belonging to common biochemical phenotypes normally had a similar pattern of adherence and VT production, but differed greatly in their pattern of antibiotic resistance, pointing to a high rate of antibiotic-resistance transfer among these isolates


Assuntos
Criança , Humanos , Aderência Bacteriana/genética , Técnicas de Cultura de Células/métodos , Impressões Digitais de DNA , DNA Bacteriano/análise , Diarreia/microbiologia , Farmacorresistência Bacteriana Múltipla/genética , Escherichia coli O157/classificação , Fator F/genética , Toxina Shiga I/genética , Toxina Shiga II/genética , Sorbitol
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