isolation and detection of acanthamoeba keratitis in rural water sources of Arak, Iran

Acanthamoeba species are free-living protozoa that can be isolated from all environments. They can bring about different diseases in healthy individuals and immune suppressed patients, for example, Granulornatous Amoebic Encephalitis [GAE], Acanthamoeba Keratitis [AK], Cutaneous and Nasopharyngeal infections. The aim of this study was to 1 evaluate the Acanthamoeba prevalence in rural water sources of Markazi province. In this cross sectional study, 54 water samples were j collected from 36 villages of Markus province. First, the Samples were filtered 1 by filter paper [watchman 42]. Next, the filtered paper were placed in page I saline solution and centrifuged. Then, the obtained sediment was cultured on non-nutrient agar [NNA] plates overlaid with heat-killed Escherichia colL After that, the provided smear [after 4-7 days] stained with Geimsa. The samples were positive [33; 61.11%] and negative [21; 38.89%] for Acanthamoeba cyst. The best result for isolation of Acanthamoeba cysts was obtained after shaking of filter paper. A high percentage of rural water sources have been contaminated with Acanthamoeba which can be the major factor in causing human infections. Therefore, some effective methods are required to prevent from water sources contamination

Knowledge, attitude and practice of native Gilanian students Shaheed Beheshti University of Medical Sciences, about fascioliasis disease

Health care professional work to solve health problems of the society. This is the duty of health system to identify the gaps in health professionals' competence and asks the medical school to fill the gap. We took one important health problem Fascioliasis and determined the amount of knowledge, attitude and practices of Gilani students studying at Medical University of Schaheed Beheshti, about Fascioliasis disease. This study was carried on 135 male and female Gilani students studying at different degrees in the age range of 18 to 37. The data was gathered by questionnaire surveys and then validated through content validity and reliability by test-retest method. Data analysis was carried out by SPSS and Microsoft Excel soft wares. To examine the correlation between variables, the T-Test and variance analysis test was used. The research findings revealed that the community under study has an average practice. In all three parameters, however, the average scores of females are greater than males. It is recommended to revise the educational programs for the students so that the students of every region become familiar with health problems of their regions