Effect of disinfectant solutions on a denture base acrylic resin

El objetivo de este estudio fue evaluar la microdureza, rugosidad y pérdida de masa de resinas acrílicas para base de dentadura, después de su exposición a soluciones desinfectantes, in vitro. Cuarenta especimenes de resina acrílica para base de dentadura (Clássico, Brasil) fueron confeccionados y asignados randomizadamente a 4 grupos (n=10) según la solución desinfectante: G1: control, almacenado en agua destilada a 37 °C; G2: 1 por ciento hipoclorito de sodio; G3: 2 por ciento Glutaraldehído; G4: 4 por ciento clorhexidina. Los especimenes fueroninmersos por 60 minutos en la solución correspondiente. Posteriormente, los especimenes fueron analizados antes ydespués de cada inmersión usando un rugosímetro (Surfcorder SE 1700 KOZAKALAB), un microdurómetro FM-700 (Future Tech), un microscopio Electrónico de Barrido (MEB) (DSM 962-ZEISS) y una escala digital. Después de los procedimientos de desinfección, los valores promedios obtenidos de cada análisis fueron evaluados estadísticamente. Las resinas para base de dentadura pueden ser vulnerables a los cambios de superficie cuando son inmersos en soluciones desinfectantes.

Effects of biologic tissue adhesives on retinal pigment epithelial chemotaxis: a comparative in vitro study

Using a modified Boyden chamber technique, the chemotactic activities of three biologic tissue adhesives [Fibronectin [FN], cell tak [CT] and matrigel [MAT]] were studied. It was found that, FN as a biologic adhesive at a concentration of 50 mug/ml, stimulated the migration of human retinal pigment epithelial [HRPE] cells, but not a great extent. Cell tak [CT] stimulated chemotaxis of HRPE cells much more effectively than FN. However, CT was combined with growth factor [as FN at a concentration of 1.5 mug/ml] or transforming growth factor-beta [TGF-B at a concentration of 10 pg/ml] it did not enhance or diminish the migration of HRPE cells. Matrigel [MAT] by itself had very little chemotactic activity. However, the migration of HRPE cells was greatly enhanced when FN and/or TGPB were added as growth factors. The study concluded that, biologic tissue adhesives exhibit variable chemotactic activities, these were maximum with cell tak, moderate with fibronectin and minimal with matrigel. However, the presence of serum components might modify this activity. The results of this preliminary study demonstrated another important feature of a biologically active tissue adhesive when used as a chorioretinal glue, its potential RPE chemotactic activity. This entails a definite clinical significance during the management of complicated cases of retinal detachment [RD], especially those associated with proliferative vitreoretinopathy [PVR] or the presence of serum components within the vitreous cavity after trauma or breakdown of the blood retinal barrier [BRB]