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Background and Aim: Nowadays, the importance of pathogenicity of non-tuberculosis mycobacteria is well known. Generally, this group, in addition to the respiratory system can cause lymph nodes, skin, soft tissue and bone disorders. Identification of Mycobacterium by culture and biochemical tests may take several weeks and may not be useful for definitive diagnosis. PCR-RFLP [PRA] technique of the hsp65 gene using Haelll and BstEII enzymes is a precise method for species differentiation, in comparison to phenotypic methods. It is a quick and inexpensive method for detection of mycobacterial species
This study aimed to assess the prevalence of non-tuberculous mycobacteria isolated from the patients referring to tuberculosis center [TB] of Kashan University of Medical Sciences
Material and Method: The study included 106 patients who had been referring to TB Center of Kashan University of Medical Sciences, from 1391 to 1394. The samples were tested by biochemical diagnostic tests. At the same time identification of the strains was made by use of PRA
Amplification of 441-bp fragment was performed by PRA for detection ofhsp65 gene
The PCR products were digested with Haelll and Bstell enzymes and analysis was performed on the basis of electrophoresis
Results: Molecular analysis showed non-tuberculosis mycobacteria in 4[8.3%] sputum samples,i.e. one positive sample [0.9 %] for every one of the following strains: M abscessus, M. senegalense, M.fortuitum and M.kansasii
Conclusion: The results of this study showed that some cases of tuberculosis in Kashan are due to non-tuberculosis mycobacteria. Also use of PRA analysis of hsp65 gene for clinical specimens is a rapid and useful tool for identification of species of mycobacterium which is helpful for early diagnosis, treatment and control of tuberculosis
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There are different surgical procedures for circumcision. Although the classic method is the most common one, it takes more time and causes more pain and complications compared to other methods. The purpose of this study was to compare the complications and the time taken for performing the surgery for Gomco and classic methods of circumcision. This case-control study was performed on 80 neonates referred to the outpatient operating room in Shahid Beheshti hospital for circumcision in 2011 and were divided into two equal groups: one group underwent the classic method and another, the Gomco method. The complications were assessed in two phases: during the surgery by counting bloody gauze pads and one week thereafter using the objective methods, then they were analyzed statistically. The mean age of neonates was 24.8 +/- 12.37 days and no significant difference was seen between two groups. The mean operation duration in the classic method [24.3 +/- 2.5 min] was approximately two times greater than that of the Gomco method [13.3 +/- 2.3 min]. Moreover, the amount of bleeding in the classic method [4.1 +/- 0.6 cc] was over three times greater than that of the Gomco one [1.3 +/- 0.7 cc]. No cases of infection were seen in both groups. Results show that the Gomco method takes less time and causes less pain and fewer complications compared to the classic method and may also be a suitable alternative to the classic method
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To report and evaluate the characteristics of patients referred to the emergency department of Labbafinejad Medical Center with work-related eye injuries during 2005. In this descriptive study, an available sample of patients with work-related eye injures were assessed. Demographic characteristics, causes of ocular trauma, and use of work safety devices were main evaluated variables. Overall, 99 subjects [96% male] with mean age of 31.76?10.55 years were enrolled. Mean time interval between the accident and referral to eye emergency was 2.46 +/- 4.46 days [0-30 days]. Of these, 66.6% came to the emergency ward on the first day after the incident and 37.1% underwent an eye examination at this center for the first time. The most unsafe activities were grinding [21.4%], welding [20.4%], hammering work [14.3%] and manual work [10.2%]. Metal industrial [50%], constructional [17.3%] and mechanical [8.2%] workers had the highest risk for eye injury among different occupations. The major consequence of eye injuries was corneal foreign bodies [72.7%]. Of injured patients, only 14 persons [14.1%] had passed job safety educational courses and 93 persons [93.9%] had not used any eye protection device. Most injured patients were young men workers using no safety devices; therefore it seems that occupational safety efforts including eye protection education and training programs in this context is insufficient
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Observational studies are not often reported in detail and clear enough, so that assessment of the strengths and weaknesses of these studies is not straightforward. To improve the reporting of observational studies, a checklist of items called 'Strengthening the Reporting of Observational Studies in Epidemiology' [STROBE] was developed by some experts in October 2007. The aim of this study was to assess the quality of reporting of observational studies before STROBE statement. We included randomly sixty cohort studies published in six important international journals until October 2007. Then, we used STROBE checklist to assess the strengths and weaknesses of these included studies. On average, more than 81% [95% Cl: 77%-87%] of included studies pointed to 43 items of aim of this study. The most reported [100%] items were "scientific background" and "rationale for the investigation" and the less reported [30%] item was "flow chart". Although, the quality of reported cohort studies' results was acceptable, the type of study, journal and date of publication could influence on the quality of observational studies