[Effect of chemical and mechanical titanium surface treatment on fibroblast attachment and viability]

Cell-titanium interactions are crucial to the clinical success of bone and dental implants. Mechanical or chemical surface treatment can help the cell attachment to implant surface. The purpose of this study was to investigate the effect of two distinct surface treatments [sandblasted and acid-etched] on fibroblasts cell attachment and viability. In this experimental study the fibroblasts behavior was analyzed on three different titanium surfaces: sand blasted [SB], Acid-Etched [AE] and pure commercial titanium [PCT], in three groups, [N=10]. Scanning electron microscopy [SEM] showed distinct micro topographies. Cell morphology and initial attachment were evaluated by SEM. Cell viability was measured by means of a 3- [4, 5-dimethylthiazol-2-yl] -2, 5-diphenyltetrazolium bromide [MTT] assay. The number of viable cell counts were compared using one way ANOVA and post hoc Tukey Test. SEM observation revealed drastic differences in surface micro topography, with a higher cell density on [SB] than [AE] and [PCT]. Cell attachments were much better in the sand-blasted group than others. Cell viability recorded by the sand-blasted group [1.28 +/- 0.58] was significantly better than acid-etched [0.95 +/- 0.23] and pure commercial titanium [1.16 +/- 0.17]. [P<.05] Implant surface treatments influence the attachment and viability of fibroblasts [L-929], and sand-blasted treatment seems to be the most favorable surface to compare with acid-etched and pure commercial titanium surface

Detection of pestivirus contamination in cellcultures by nested-PCR

In this study a nested-PCR assay was optimized for detection of two BVDVbiotype of NADL strain. Apart of 5' non-coding region of virus, 249 bp in size, was amplified in RT-PCR. PCR product was cloned in a pTZ57R/T vector and sequencing results confirmed the specificity of the test. Internal primers were designed and a 155 bp DNAfragment was amplified in nested-PCR. The 4 sensitivity of RT-PCR and nested-PCR for detection of virus in cell culture were found to be 10 2 TCID50 and 10 TCID50, respectively. Seven cell cultures were tested for BVDVcontamination using ELISA, RT-PCR and nested-PCR. Results indicate that sensitivity of molecular tests for detection of virus in cell culture samples is higher than ELISA