HPLC assay of lidocaine hydrochloride and prednisolone acetate in anti-hemorrhoidal preparations

Prednisolone acetate and lidocaine hydrochloride are commonly incorporated among other drugs in most antihemorrhoidal ointments and suppositories. This study described HPLC method for the determination of both drugs in these dosage forms. The procedure involves separation and quantitation of each drug without interference from other commonly present ingredients, specially ruscus. The mobile phase was a mixture of methanol, water and phosphoric acid in the ratio of 60: 40: 0.1, respectively. Benzoic acid was used as internal standard

HPLC assay of reportorial hydrochloride

This study described HPLC procedure for the determination of reproterol hydrochloride as bulk drug or in dosage forms and in presence of serum. The mobile phase is a mixture of methanol, water, tetrahydrofuran and phosphoric acid in the ratio of 60: 40: 0.1, respectively. Propranolol hydrochloride was used as a the internal standard. The developed procedure can detect down to 10 mug ml-1 reproterol hydrochloride. A linear relation was found between the peak height ratios of different drug concentrations to that of the internal standard and the drug concentrations in the range of 20-140 mug ml-1 with a mean percentage recovery of 100.25 +/- S.D. 1.092

High performance liquid chromatography assay of atenolol and acebutolol

High performance liquid chromatographic method for determination of the beta-adrenergic blockers, atenolol and acebutolol was described. The method involved separation on a C[18] column, using propyl paraben as internal standard and a mixture of water, methanol, acetonitrile and phosphoric acid as eluent in different ratios for each drug. They have been detected at 235 nm. The mean percentage recoveries of atenolol [20-120 ug ml-1] and acebutolol [10-100 ug ml-1] were 99.63 +/- SD 1.041 and 100.43 +/- SD 0.78, respectively. The method has been applied for the determination of the two drugs in their dosage forms

Fluorometric determination of chloramphenicol

A simple, accurate spectrofluorometric method for determination of chloramphenicol is presented. The method depends on reduction of the nitro group of chloramphenicol with zinc in acid medium. When the reduction product was reacted with salicylaldehyde solution, a highly fluorogenic reaction product was produced having excitation and emission wavelengths at 386 and 495 nm respectively. The measured fluorescence was found to be linear in the concentration range of 5X10[-7] up to 5X10[-6] mg/ml. The method was applied for the determination of chloramphenicol in its pharmaceutical preparations. The B.P. 1980 method was applied for comparing the results

Colorimetric determination of hydrocortisone

Hydrocortisone is an anti-inflammatory, immunosuppressive and antiallergenic drug. The present work deals with the development of a colorimetric procedure for its determination. The 17 alpha ketol group of hydrocortisone was selectively oxidized with potassium periodate into formaldehyde which was colorimetrically determined using 4-amino-3-hydrazino-5-metcapto-1,2,4-triazole [AHMT]. The optimal conditions of the proposed procedure are presented. The produced color obeys Beer's law in a concentration range of 0.005-0.035 mg/ml. Different drugs and additives that may be present with hydrocortisone in its different dosage forms did not interefere in the proposed procedure except chloramphenicol and ampicillin. Some pharmaceutical preparations containing hydrocortisone were analysed by this procedure and the results were compared with those obtained by the official tetrazolium procedure; USP XIX