Evaluation of some biochemical changes in diabetic patients

The present study consisted of 50 subjects were classified into three groups; Group [GI] Control group consisted of 10 clinically healthy adult subjects of both sexes free from any liver, kidney or cardiovascular diseases. Group [GII] diabetes mellitus type 1 consisted of 20 patients of both sexes. Group [GIII] diabetes mellitus type 1 with nephropathy consisted of 20 patients of both sexes. All subjects were undergo to the following investigated parameters; Ascorbic acid [vitamin C], Catalase, Total antioxidant capacity, Aldolase and Pyruvate kinase enzyme. vitamins C, catalase, total antioxidant capacity enzymes were highly significant decreased [P < 0. 01] in diabetes mellitus type 1 [GII] and diabetes mellitus type 1 with nephropathy [GIII] when compared to the control group. Adolase activity was highly significant increased [P < 0. 01] in diabetes mellitus type 1 with nephropathy [GIII] when compared to the control group. Pyruvate Kinase activity was highly significant increased [P < 0. 01] in diabetes mellitus type 1 [GII] when compared to the control group. The antioxidant and enzymes can be used for follow up in patients suffering from diabetes mellitus type 1 and predict other complications

Changes of P21 and Bcl-2 during induction of bladder and/or bladder carcinogenesis

The effect of E. coli infection in association with dibutylamine and sodium nitrate in induction of bladder inflammation and /or bladder carcinogenesis were investigated in 150 male albino rats, divided into five groups, as follows: The first group [G1] infected by E. coli, The second group [G2], given nitrosamine precursors in the diet, The third group[G3] infected by E. coli and given nitrosamine precursors in the diet The fourth group[G4] infected by E. coli, given nitrosamine precursors in the diet and received standard diet containing 1% Curcumin powder mixed in the diet and the fifth group [G5], served as control group. The worst histopathological changes are in G3. A highly significant decrease in the mean cell lysate level of p21 was found in different studied groups[G1,G2 and G3] especially in[G3] where P values in these groups when compared to control group were [P < 0.0001] . In curcumin treated group [G4], there were downregulation in the mean cell lysate level of p21 gene when compared to control group but higher than other studied groups [G1, G2 and G3]. A highly significant increase in the mean serum level of Bcl-2 was found in different studied groups [G1,G2 and G3] especially in [G3] where P values in these groups [G1,G2,G3] when compared to control group were [P <0.0001]. [G4] showed higher Bcl-2 serum level when compared to control group, this difference was insignificant but this level is lower than other studied groups [G1,G2 and G3]. The results in the present study indicate that both P21 and Bcl-2 can be used as biological markers in the diagnosis of bladder cancer. Curcumin have the ability to overcome the decrease in p21 WAF1/CIP1 protein and the increase of Bcl-2 protein and reduce the induction of carcinogenic effect

Immunoglobulins, cortisol and acth levels in sera of Egyptian iddm

Sera of 75 diabetic patients [IDDM] were analyzed for IgG, IgA, IgM, IgE, cortisol and ACTH levels. The study revealed a significant increase in concentrations of IgG, IgA, IgM and IgE in all patients. In addition, serum cortisol and ACTH concentrations showed a significant increase in GDRF and GDM, while in diabetic group no such finding was observed. So, serum levels of cortisol and ACTH besides the above mentioned parameters could be a diagnostic value in differentiate IDDM and the accompanying complications like renal failure as well as myocardial infarction

Creatine kinase, lactate dehydrogenase, cortisol and ACTH in chronic renal failure and kidney transplanted patients

Creatine kinase [CPK], lactate dehydrogenase [LDH], cortisol and adrenocorticotropic hormone [ACTH] were evaluated in 75 subjects, classified into 3 groups: Normal healthy 25 cases as a control group [I], chronic renal failure patients [CRF] 25 cases group [II], and renal transplanted patients [RT] 25 cases group [III]. The mean level of serum CPK activity in control group was 98.76 +/- 26.67 U/L, while in chronic renal failure patients and renal transplanted subjects the mean level were 205.80 +/- 37.12 U/L and 173.91 +/- 39.36 U/L, respectively, these data were significantly higher than the control group. The mean level of serum LDH activity in control group was 313.14 +/- 39.37 U/L, while in chronic renal failure and renal transplanted subjects the mean level of serum LDH were increased significantly to be 389.18 +/- 52.40 U/L and 387.35 +/- 52.35 U/L, respectively. The mean level of serum cortisol concentration in control group was 112.67 +/- 35.92 ng/ml, while in chronic renal failure patients and renal transplanted subjects the mean of serum cortisol concentration increased significantly to be 227.36 +/- 52.04 ng/ml and 165.52 +/- 44.34 ng/ml, respectively. The mean level of plasma ACTH concentration in control group was found to be 8.43 +/- 5.15 pg/ml, while in renal transplantation patients ACTH increased significantly to be 16.9 +/- 10 pg/ml than the control group

Glutamate dehydrogenase and some biochemical parameters in chronic renal failure and kidney transplanted patients

The serum activity of glutamate dehydrogenase [GLDH] was measured spectrophotometrically in 75 cases, normal healthy 25 cases as a control group [I], chronic renal failure 25 cases group [II] and renal transplantation patients 25 cases group [III]. The mean level of GLDH activity in chronic renal failure [12.55 U/L] was significantly higher than the control group [1.58 U/L], and also, higher than the corresponding mean level of renal transplantation patients [2.54 U/L]. The result indicated that evaluation of serum GLDH activity has diagnostic potential