Production of alpha-amylase from bacillus lentus

A number of nutritional factors affecting growth and alpha-amylase by amylase by Bacillus lentus were investigated. The production of enzyme increased with increase of temperature reaching its maximum between 40°C - 45°C after 60h incubation when cultivated in a medium with 30 g/1 starch as carbon source. Supplementation of the medium with different carbon sources did not enhance either enzyme activity or growth. Of the nitrogen sources examined a mixture of peptone, yeast and beef extracts stimulated growth and yielded the highest enzyme production

On the production of alpha-amylase by free and immobilized cultures of bacillus lentus

The effect of natural additive, amino acids, vitamins, surfactants on the production of alpha-amylase by Bacillus lentus was studied. Also the production of alpha-amylase was followed with cells of B. lentus immobillized by entrapment in Ca-alginate [2%] in static, shaked and insemi-continuous cultures, and by adsorption on granular clay