Quantitative determination of xipamide in pure form and in tablets through complexation with iron [III] chloride

Two simple, accurate, and reproducible spectrophotometric methods for the determination of Xipamide [XIP] in pure form and in pharmaceutical preparations were described. The first method was based on the formation of orange complex between iron [III] chloride and the investigated drug. The method permits the determination of XIP over a concentration range 20-160 micro g ml[-1] at 490 nm. The second method was developed by formation of ternary complex by reaction of the drug with Fe [III] chloride and ophenanthroline [phen] in the presence of sodium lauryl sulphate as surfactant and measuring the absorbance at 440 nm. The method obeys Beer's law over the concentration range of 10-80 micro g ml[-1]. The factors affecting the formation of complexes were studied and optimized. The relative standard deviations of these methods were less than 1.0% and their detection limits were 2.75-4.45 micro g ml[-1]. The methods have been successfully applied for the determination of the studied drug in its pharmaceutical tablets and the results obtained were in good agreement with those obtained by the reference method

Correlation between peripheral hormonal profile and mid-luteal endometrial histology in patients with unexplained infertility

This study investigates the relationship between mid luteal endometrial histology and serum levels of progesterone and estradiol, also the relationship between endometrial dating and serum levels of prolactin, FSH, LH, T3 and T4. The results revealed that patients having progesterone values less than 4.6 mg/ml, estradiol values less than 86 pg/ml showed proliferative or anovular pattern. On the other hand 100% of patients with progesterone values over 9.9 ng/ml and 84% of patients having estradiol over 86 pg/ml, showed secretory activity. There was a good correlation between endometrial histology and progesterone and estradiol, specially progesterone which showed 100% correlation