Isolation of serratia liquefaciens from breeding farms, and its prevalence in human being

Serratia liquefaciens, a member of the Klebsiellae, is an uncommon pathogen, It's related member, S. marcescens acquired an attention due to its prevalence among hospitalised patients. S. liquefaciens is now recognised to be one of the most important causes of animal septicaemia. It may cause epidemics in chickens, workers dealing with infected chickens were found to be highly colonised by this organism, of 138 workers investigated 25 [18.12%] were found to harbour the organism: out of them 58 workers developed clinical disease and the organism was isolated from 16 patients [27.59%], the infection is an occupational hazard when compared to other patients not exposed to chicken. The difference is significant in exposed workers. In birds it was isolated from 84 samples from dead birds [76.36%], and from 41 samples from sick birds [45.56%]. Also isolated from outer Egg-shell from infected farms, out of 60 samples examined 15 samples were positive [25%]. The water supply were examined daily for 10 days, and the organism was isolated from 8 samples out of 30 samples examined [26.6%]The great danger in infection by this organism is its high resistance to many antibiotics, which may emerge from repeated unwise use of antibiotics as a prophylactic measure for chicken breeding. Ampicillin, gentamycin and tobramycin were ineffective, Amikacin and Nalidexic acid were poorly effective, but the organism was strongly sensitive to imipenem, ciprofloxacin and lincospectine

Application of mycoplasma gallisepticum DNA probes by using dot blot hybridization

Application of DNA M. gallisepticum DNA probe as rapid procedure for accurete diagnosis of M. galllisepticum infection in chicken farms. Dot blot hybridisation to the six M. gallisephcum, one M. Synoviae, one gallinarum one. M. Iowe, F. 810 [vaccine strain] and two DNAs, of S6andF810. This technique was rapid and accurete in dectection of M. gallisepticum and easier to differentiate between avian Mycoplasmal species and strain. The quantity of DNAS is very important in clearence of colour indicator and small quantily less than 5x10[8] Da can interfere with the obtained results. Probes provide a high degree of specificity and sensitivity that is equal to or greater than that seen when using the current methods of indirect detection of acian mycoplasma as. e. q. detection of specific antibodies

Some bacterial pathogenies transmitted by fresh water fish to duckling

Four hundreds fish [200 Telapia nilotica and 200 Common carp] were collected from different fish farms at Sharkia province. These fish were divided into 4 equal groups. The first group was subjects to clinic and bacteriologic examination as soon as they were collected, to determine the percentage of pathogens in different fish farms. The groups 2, 3 and 4 were subjected to continuous clean water, freezing and drying then grinding respectively to detect the effect of these treatments on the incidence of the pathogens on treated fished. Eighty one day old duckling were experimentally infected by the isolated bacteria through different routes of inoculation, to determine the pathogenicity of some strains from fish to duckling. The results showed that high percentage of E. coli, Arizona, Str. foecalis, A. hydrophila, Shigella and Salmonella were appeared specially in private fish farms and San-EI-Hagar fish hatchery. The most common signs on infected fished appeared as dark black colouration emaciation and ascitis. Fifteen days duration were not sufficient to purify the fishes from the pathogenic bacteria, Str. foecalis resist freezing at- 20°C for 10 days and drying at 80 degree destroyed all the pathogenic bacterial agents. Enterobacteria, Str. faecales and A. hydrophila were highly pathogenic to duckling causing, Septicemia, emaciation and mortalities about 50, 75 and 100% respectively. Histopathologic alteration and clinical signs on infected duckling with A. hydrophila and Str. foecalis were recorded