Diagnosis of echinococcosis [hydatidosis] using dot-ELISA

Background: Cystic echinococcosis [CE] is a complex, chronic and neglected disease caused by the larval stage of Echinococcus granulosus. The effects of this neglection have a powerful impact in remote rural areas whose population has no chances of being diagnosed and treated correctly without leaving their works and travelling long distances, sometimes taking days to reach the closest medical center. The present study was designed to evaluate the diagnostic efficacy of purified polyclonal antibody [PAbs] raised against Echinococcus granulosus 50 and 31 kD proteins for detection of circulating hydatid antigen using dot ELISA

Materials and methods: The previous proteins from sheep and camel lungs was purified by ammonium sulfate and caprylic acid. The purified protein injected in Newzealand rabbits to raise specific polyclonal antibodies [pAb] against E. granulosus. Detection of 50 and 31 kD proteins in serum by dot-ELISA gave a sensitivity of 92.9%, a specificity of 95%

Conclusion: Dot-ELISA techniques emerge to be adequately sensitive assays for the diagnosis of human echinococcosis using cathepsin B antigen

Anatomical and surface morphology of hedruris lut- janenses sp. n. [nematoda: hedruridae] from the common marinewater fish lutjanus synagris in Damietta, Egypt

The description and tegumental ultrastructure of nematode Hedruris lutjanenses sp. n. collected from marine Lane Snapper fish Lutjanus synagris Linnaeus, 1758 collected at Manzala Lake, Damietta, Egypt was studied by light and Scanning Electron Microscopy [SEM]. The new species is mainly characterized by that the buccal capsule is surrounded by four pseudolapia; one small and three larger pseudolapia, each one bears a number of papillae arranged irregularly on its surface and the presence of one pair of precloacal papillae and nine pairs of postcloacal ones in males

review of genus prothogonimus Luhe, 1899 [prothogonimidae], from the Egyptian house sparrow: passer domesticus niloticus

A review of the genus Prothogonimus is given. Two species assigned to the subgenus Prothogonimus P. [Prothogonimus] vitellatus Nicoll, 1914 and P. [Prothogonimus] sp. are described. A key is presented to the subgenus Prothogonimus known so far in Africa

On aphanurus pimelopteri SP N [trematoda: hemiuridae] from the Red Sea fishes

Aphanurus pimelopteri sp. n. is described from the fish Pimelopterus tahmel caught from Al-Ghardaga, Red Sea. It differs mainly from the other species of the genus by long cranulate body shape, ovary situate in posterior third of body length, vesicula seminalis lies in mid third of body length and ventral sucker occupying almost all body width. The new species is also compared with other known species of the genus and followed by a key to distinguish the different known species of its genus

review of the cestode genus rhinebothrium linton, 1889 [tetraphyllidae], with a description of two new species of the sting ray taeniura lymma from the Red Sea

The genus Rhinebothrium is reviewed. Rhinebothrium [Rhinebothrium] ghardaguensis N. sp. and R. [Rhinebothrium] taeniuri N. sp. are described from Taeniura lymma, a cartilagnous fish caught at Al-Ghardaga, Red Sea

On pycnoporus mansouri NSp [tremetoda: lecithodendridae] from the Egyptian bat pipistrellus kuhli

A new species of the genus Pycnoporus P. mansouri is described from Pipistrellus kuhli. It is compared with other related species of the genus and a key to the genus Pycnoporus is given