RESUMO
The aim of this study is to evaluate the effect of different superovulatory regimens on embryo production of buffalo-cows in Egypt. Estrus synchronization applied on 16 multiparous Egyptian buffalo-cows divided randomize into three groups A [n=4], B [n=6] and C [n=6]. Only, thirteen buffalo cows responded in the three groups A [n=3]; B [n=4] and C [n=6]. The buffaloes showed estrus from the three groups were received a dose of 2500 IU of eCG on day 8 of the cycle. In group A [n=3] no, further superovulatory treatment was applied, whereas animals of group B [n=4] received a single dose of 2500 IU of hCG 30 h after AI. Buffaloes of group C [n=6] received the same regimen of group B in addition to i.m injection of 2.5 mg Estradiol benzoate [EB] and 50 mg progesterone [P4] 4 days before superovulatory treatments. Embryos were collected from all groups non-surgically during day 6 [around 140] h from estrus onset. The results reveals that there were a significance increase [P<0.05] for the number of corporalutea and collected embryos in group C than other treated groups. In conclusion, progesterone supplementation with estradiol benzoate 4 days before initiating the superovulation treatment seems to enhance the recovery rate in buffaloes. Further studies are needed in order to increase the number of embryos recovered per a buffalo female