External human exposure and management immune system in pathogenesis of irritable bowel syndrome

External exposed radiation may play an important role in pathogens of irritable bowel syndrome [IBS], although is thought to arise due to a combination of genetic and environmental factors. The result is dysregulated immune responses due to alteration in the gut microbiota population and the subsequent development of gut inflammation. It has recently been shown that the effect of ionizing radiation on T-cell lymphocytes might be a risk factor of IBS. This article tries to discuss the effects of low dose radiation on alternation in the population of beneficial members of the gastrointestinal tract flora. The result may be activated inflammatory response of mucosal immune status induced Toll like receptors. Lipopolysaccharides produced by most gram-negative bacteria of gastrointestinal tract play a role in the initiation and progress of the Toll like receptors in the intestines. Circulating cytokines and soluble receptors released by activated Th2 can regulate neuronal function via endocrine mechanisms. Disturbance of the cytokine-mediated interaction between cells may lead to neuronal dysfunction or sensory dysfunction, motor dysfunction, or both sensory and motor dysfunction in patient with IBS

Effect of ionizing radiation on development process of T-cell population lymphocytes in Chernobyl children

The aim of preliminary study was determined development process status of T-cell population lymphocytes in Ukrainian children after 22 years from Chernobyl accident for next feasibility study. 150 participants aged 6 to 16 years are included in three groups: Group I [n=65], 30 to 60 km from center accident at zone 3th, Group II [n=65] 60 to 90 km from same location at zone 4[th] and control group [n=20] from Kiev, 100 km from same location. Peripheral blood leukocytes from buffy coats were analyzed for T-lymphocytes population such as T-lymphocytes [CD3], T-helper [CD4] and T-cytotoxic [CD8] by roseting method using erythrocytes that conjugated with monoclonal antibody against CD3, CD4, and CD8 receptors; then CD4/CD8 ratio were calculated. Percentage of CD3 and CD4 in groups II and I decreased significantly in compared to control group at P<0.001. Percent of CD8 decreased significantly in group I compared to control group at P <0.001. CD4/ CD8 ratio decreased significantly in-group I comparison to control group at P=0.02. Leucocytes count in groups II and I have not changed significantly in comparison to control group [P=0.09,P=0.4] but in group II, it was significantly different in comparison to group I at P <0.008. Our data show that after 2 decade of Chernobyl accident, ionizing radiation may have affected the developmental processes of T-cell population