Environmental factors affecting urease production by penicillium funiculosum 258

Maximum urease synthesis was obtained by the fungus Penicillium funiculosum 258 when grown on medium containing urea as sole nitrogen source at concentration of 0.5% and 1% glucose as carbon source adjusted to pH 6 and inoculated with 10% previously prepared inoculum in shaked culture with 4: 1 air medium ratio for 3 days incubation. Urease production by the tested fungus required addition of thiamine and biotin to the culture medium at concentrations of 0.001%. The enzyme activity of the formulated medium increased from 15.3 mu/ml to 29.65% mu/ml and the enzyme productivity also increased from 3.31 to 8.97 mu/mg dry weight