Spontaneous apoptosis of endometrial tisssues in patients with endometriosis

To detect spontaneous apoptosis in eutopic and ectopic endometrial tissues obtained from patients suffering from endometriosis compared with normal eutopic endometrial tissues from healthy fertile controls, aiming to assess the possible role of apoptosis in the pathophysiology of endometriosis. A prospective study. Study group of 22 patients undergoing laparoscopy for endometriosis and a control group of 16 healthy fertile women with regular mensis and without any gynecological disorders. Spontaneous apoptosis of eutopic endometrial tissues [Obtained by Novak's curette from patients and controls] and ectopic endometrial tissues [Obtained from patients during laparoscopy by laparoscopic biopsy forsceps] was detected using ELISA - based cell death detection kit which is a highly sensitive and specific assay for apoptosis. Spontaneous apoptosis was significantly decreased [p < 0.001] in eutopic endometrium of patients with endometriosis compared with healthy controls and this decrease was not attributable to the phase of the menstrual cycle. Also, in paired samples from all patients with endometriosis, there was a significant reduction [p < 0.001] in spontaneous apoptosis in ectopic endometrium compared with the eutopic one which was not attributable to the phase of the menstrual cycle. Abnormal survival [due to lower apoptosis] of eutopic endometrial tissues results in their continuing physiologic activity after displacement into ectopic sites, giving origin to endometriosis. So, the cause and / or pathogenesis of endometriosis is at least partially because of reduced sensitivity of endometrial tissue to apoptosis

Study of apoptosis in human uterine endometrium in different phases of the menstrual cycle

Our purpose is to investigate spontaneous apoptosis in the human uterine endometrium throughout different phases of the menstrual cycle and its possible role in regulation of the human menstrual cycle. Spontaneous apoptosis in forty endometrial biopsy specimens [obtained from forty healthy, fertile women with regular menstrual cycle at different phases of the cycle] was assessed with an ELISA- based cell death detection kit which is a highly specific assay for apoptosis Spontaneous apoptosis occurs in specific population of cells in the human uterine endometrium during three different phases of the menstrual cycle: early proliferative [EP] [1.62 +/- 031 nm], late secretory [LS] [1.76 +/- 0.36 nm] and menstrual phase [M] [1.54 +/- 0.27 nm]. Apoptic changes during the menstrual cycle might play a pivotal role in the regulation of the cycle

Studies of egg drop syndrome-1976: 1- Existence of antibodies in Egyptian commercial chicken layers

Six outbreaks of severe dropped egg production and production of pale eggs, soft shelled and shell less eggs correlated with the appearance of hemagglutinating inhibiting antibodies to 127-virus are described. The outbreaks were selected from a larger number of flocks with similar problems in the field