Effects of streptozotocin-induced diabetes on rat renal function and structure

The effect of streptozotocin on renal glomerular activity was measured 1, 4 and 6 weeks after IP injection of STZ in rats. A significant increase in blood and urinary glucose levels as well as urinary volume creatinine in serum and urine and creatinine clearance were observed one week after STZ injection. These elevated parameters were maintained during the entire 6 weeks experimental period. In diabetic group there was gradual increase in the weight of the kidney with peak level after 4 weeks. Insulin treatment produced a gradual effect and by the end of the 6th week all the previously studied parameters showed a progressive decrease although still significantly higher than those of normal control group put without glucosuria

ultrastructural study of the effect of commonly used oils on the liver of adult rat

An electron microscopic study has been done on the liver of albino rat to compare the effect of injection of 3 commonly used vegetable oils of [cotton seed, soy bean and corn]. 12 albino rats were used in this study, divided into 4 groups of 3 rats each. Group I served as control. Groups II, III and IV were forcedly fed on the above mentioned three vegetable oils respectively in group II, cotton seed oil showed the least effect in the form of small areas of degenerated cytoplasm and the presence of secondary lysosomes. In group III, soy bean resulted in patchy hepatocytic cytoplasmic vacuolations, degenerated mitochondria, fragmented rough endoplasmic reticulum, accumulation of fat droplets and increased number of secondary lysosomes. In group IV, corn oil caused degeneration of mitochondria, accumulation of fat droplets, increased number of secondary lysosomes and the appearance of lamellated bodies in the cytoplasm. These results show that cotton seed oil is the most safe oil to use for long periods