RESUMO
In order to overcome the false negative diagnosis of infection with C. philippinensis in the absence of eggs in stool, coproantigen prepared from the stools of infected patients was evaluated serologically. This antigen was able to detect anti-Capillaria antibodies in the sera of infected cases at the same OD level produced with Capillaria crude worm antigen using indirect ELISA technique. C. Philippinensis coproantigen did not cross-react with the sera from patients with Schistosomiasis mansoni, Fascioliasis or Strongyloidiasis at 1:00 serum dilution. Laboratory-prepared hyperimmune sera versus crude worm antigen of C. philippinensis succeeded in capturing Capillaria antigen prepared from the stools of infected patients and did not cross-react with the coproantigens prepared from the stool samples of cases infected with S. Mansoni or Fasciola using sandwich ELISA technique
Assuntos
Antígenos/sangue , Extratos de Tecidos , Infecções por Nematoides , Fezes , Ensaio de Imunoadsorção Enzimática , Infecções por Enoplida , Antígenos de HelmintosRESUMO
The seroprevalence rates of human toxocarosis figured up to 24.5% versus Toxocara canis and 22.1% versus Toxocara vitulorum in some selected localities in Egypt, using enzyme linked immunosorbent assay technique. Toxocara vitulorum larvae in experimentally infected mice and rats follow the same manner of migration previously described for T.canis with more adaptation to mice than rats as paratenic host. This study clarifies the role of rodents in maintaining Toxocara larvae and spread of infection to other host