RESUMO
A total of thirty subjects was selected. For each individual subject, 3 impressions of maxillary arch were made using alginate. Impressions were divided into three categories. First impression (Group 1) was made without using any preprocedural mouth rinse. After the impression was made, a sterile cotton swab was wiped over the impression surface and sent for culture sensitivity test. This swab acted as the control group. The swab was then run over the Blood Agar culture medium. The incubation of culture-medium plates was done for 24 hrs. at 37 °C. From the culture plate, smear was taken to examine bacterial growth and the colony forming units were counted on the slide. 24 hours after initial impression, the second impression (Group 2) was made after making the subject rinse with aloe vera for 30 seconds. After 48 hours after Group 1, third impression (Group 3) of the same subject was made, making the subject rinse with 0.2% chlorhexidine digluconate solution for 30 seconds. Rest of the steps were same for both the impressions as the first impression.Data was recorded and analysis for difference in the bacterial count between Group 1, Group 2 and Group 3 was done using ANOVA with post-hoc Bonferroni test from SPSS version 18. ResultsThe result showed an overall significant difference in the mean CFU among the three groups. Post-hoc test showed that group 1 had significantly higher mean CFU (7.54) than group 2 (2.98) and 3 (1.82). The least bacterial count was seen in the samples in which chlorhexidine digluconate was used as a mouth rinse before making the impression. The mean percentage reduction in bacterial count was 60.33 in the group for which aloe vera was used and 75.81 in the chlorhexidine digluconate group. Conclusions0.2 % chlorhexidine digluconate when used as preprocedural mouth rinse for 30 seconds markedly reduces the bacterial growth of aerobic culture. Although Aloe vera does have an antibacterial effect when used as preprocedural mouth rinses when compared with 0.2 % chlorhexidine digluconate, it is a weaker disinfectant. The antibacterial activity of 0.2 % chlorhexidine digluconate is more effective than aloe vera (99.9 %) when used as preprocedural mouth rinse.
RESUMO
Aloe vera and chlorhexidine are traditionally used as disinfectants of irreversible hydrocolloids. Most popular among irreversible hydrocolloids is alginate which is most commonly used in dentistry. We wanted to compare and evaluate the efficacy of aloe vera and 0.2% chlorhexidine as an internal disinfectant.METHODSFor each subject three impressions of the upper arch were made using irreversible hydrocolloid impression material i.e. Alginate (Zelgan 2002). After the impression was made, a sterile cotton swab was used and wiped over the impression surface from the center of the palate and was sent for culture sensitivity test. This swab acted as the control group (Group 1). The swab was then placed in normal saline transport medium and was sent for culture sensitivity test to the Gyan Pathology Lab, Kanpur. The swab was then run over the Blood Agar culture media. Incubation of the culture medium plates was done at 37°C for 24 hrs. From the culture plate, smear was taken to examine bacterial growth on a slide, semi-quantitatively, under light microscope, and the colony forming units were then counted. 24 hours after initial impression, second impression (Group 2) was made by mixing alginate with 99.9% aloe vera. 48 hours after the first impression, the third impression (Group 3) of the same subject was made after mixing alginate with 0.2% chlorhexidine digluconate. Remaining procedural steps were similar for second and third impressions as the first impression. Bacterial count was recorded and analysed for variation between group 1, group 2 and group 3 using Repeated Measures ANOVA with post-hoc Bonferroni test from SPSS version 18.RESULTSThis study showed that using 99.9% concentration of Aloe Vera as an internal disinfectant to manipulate irreversible hydrocolloid impression material did show a reduction (65.96%) in growth of aerobic bacteria. However, the decrease was significantly less when compared with chlorhexidine.CONCLUSIONSAmongst the two disinfecting agents used in this study, 0.2% chlorhexidine digluconate has shown to be more effective disinfecting agent than Aloe Vera.