[Nanotechnology is the new method for rapid detection of vibrio cholerae]

Backgrounds and Objectives: Rapid identification of Vibrio cholera is important in epidemics and bioterrorism wars. On the other hand, the conventional method is time consuming and costly. The aim of this study was to develop a rapid, inexpensive and high sensitive method based on magnetic nanoparticles for trapping bacterial DNA

Materials and Methods: In this study, biotinalated probes were used for trapping Vibrio cholerae DNA. Finally designed primers were used to detect bacteria

Results: According to PCR identified results showed that only Vibrio cholera were identified, and; concluded that the biotinalated probe is specific for bacterium Vibrio choleraecholera

Conclusions: According to the findings of this study, Sensitive and specific detection of the bacterium Vibrio cholerae is assessed and validated with this method efficiently. And in terms of time and at lowest costs are introduced an efficient method

Curcumin-loaded chitosan tripolyphosphate nanoparticles as a safe, natural and effective antibiotic inhibits the infection of Staphylococcus aureus and Pseudomonas aeruginosa in vivo

Curcumin as a yellow natural compound extracted from turmeric root is known it as an antibacterial agent. One of the nanoparticles ability is to decrease the defects of usual drug delivery systems. Chitosan is a low toxic, biodegradable, biocompatible and safe polymer which is used in production of nanoparticles. Nanoparticles like chitosan-tripolyphosphate [TPP] are able to increase antibacterial properties of curcumin. Curcumin-loaded chitosan-TPP nanoparticles containing chitosan, curcumin and TPP salt were synthesized by ionotropic gelation methods. First, the skin of anesthetized mice was inoculated with staphylococcus aureus and pseudomonas aeruginosa suspension. Then the infected mice were treated with curcumin-loaded chitosan-TPP nanoparticles for 3 days. Following that, antibacterial characteristics of the mice treated with curcumin-loaded chitosan- TPP nanoparticles were evaluated by bacterial culture of these mice. Our results showed the size of 160 +/- 10 nm and the charge of +7 +/- 2 mV in curcumin-loaded chitosan-TPP nanoparticles. These nanoparticles were also spiral shape. The encapsulation efficiency of curcumin in chitosan-TPP nanoparticles was 75 +/- 2%. Bacterial culture showed that curcumin-loaded chitosan-TPP nanoparticles inhibited staphylococcus aureus and pseudomonas aeruginosa growth. Our study demonstrated that curcumin-loaded chitosan-TPP nanoparticles can be utilized as a potent agent in treatment of Staphylococcus aureus and Pseudomonas aeruginosa infections

Cloning and expression of coxsakievirus B3 viral protein-1 in E. coli

Viral protein-1 [VP1] is a major capsid protein of Coxsakievirus B3 [CVB3] that plays an important role in directing viruses towards permissive cells and acts as a main antigenic site of the virus in eliciting of host immune response, hence it seems VP1 can be considered as a vaccine candidate against CVB3 infection. In this study, cDNA of VP1 was prepared, cloned into pET expression vector and the recombinant protein [VP1] was over expressed in E. coli. The viruses were grown in suspension cultures of Vero cells with an input virus multiplicity of 10-50 plaque-forming units/cell. After observing complete cytopathic effect, the total RNA [cells and virus] was prepared for RT-PCR and by using specific primers, VP1 cDNA was amplified and ligated into pET vectors [32 a and 28 a]. The recombinant vector was transferred into competent E. coli [BL-21] and after selection of proper colony, which carried correct cDNA within the vector; cells were cultured and induced with isopropyl B-D-thiogalactopyranoside, in order to express protein [VP1]. The cultures were tested for presence of VP1 by SDS-PAGE and Western-Blotting analysis. Molecular techniques such as PCR which showed exact defined size of the VP1 [819 bp], restriction digestion and finally immunoblot analysis of over expressed protein; all confirmed the correct cloning and expression of VP1 in this research. In this research, full length of VP1 as major capsid protein of CVB3 was over expressed in E. coli which, can be used for further studies, including neutralizing antibody production against CVB3