RESUMO
OBJECTIVE: To determine and isolate causative organisms in the bacterial keratitis and their sensitivity and resistance to different antibacterial agents
MATERIAL AND METHODS: This was a prospective observational study containing a total of 80 patients [study participants] conducted from March 2011 to October 2012 at Ophthalmology Department of Liaquat University Eye Hospital Hyderabad. After taking informed consent, bacterial isolation procedures were done as: a corneal smear was taken after topical anesthesia, obtained by application of a drop of single dose unit; Proparacaine hydrochloride 0.5% [Alcon, Belgium]. A portion of each scrapping was examined microscopically for the presence of bacteria by using Gram staining and the isolated bacteria were tested for their sensitivity and resistance against the different antibiotics
RESULTS: In this study, male were found in the majority and mostly patients were from rural areas. Mostly causative organisms isolated from the bacterial keratitis were Staphylococcus aureus and Staphylococcus epidermidis among the gram positive organisms and Pseudomonas among the gram negative organisms, different types of the antibiotics as Cefazolin, Tobramycin, Ofloxacin, Ciprofloxacin and the Norfloxacin were tried and showed the sensitivity with the percentage of 80%,75.0%,78.75%,73.75% and 72.5%, respectively
CONCLUSION: Mostly causative organisms isolated from the bacterial keratitis were staphylococcus aureus and staphylococcus epidermidis among the gram positive organisms and pseudomonas among the gram negative organisms, different types of the antibiotics as Cefazolin, Tobramycin, Ofloxacin, Ciprofloxacin and the Norfloxacin were tried and showed good response regarding sensitivity