[Comparision of antifungal effect of fluconazole alone and in combination with nanosilver particles against candida species isolated from chronic candidal vulvovaginitis]

Candidal vulvovaginitis is a female genital infection that is occured by the over growth of candida species and specially Candida - albicans and occasionally it appears as recurrent and chronic and resist to therapy. Fluconazole is one of the current drugs that is used in treatment of this disease and sometimes resistance is observed to this. Therefore indirection of making the therapy better we decided to investigate the activitiy of fluconazole in combination with silver nanoparticles against candida species isolated from chronic and recurrent candidal vaginitis. This was an experimental study with convenient sampling that was performed on 30 patients. All specimens were examined in direct microscopy, culturingand differential tests to identify different candida species from each other such as culture on candida chrom agar, germ tube, temperature test and sugar assimilation with API. Then the antifungal effects of fluconazole and silver nanoparticles, each of them alone and in combination with each other, were examined .Findings were described on the base of logestic regression and man-vitni exam. In our study 30 specimens of chronic genital candidiasis were diagnosed with isolated agents Candida albicans, glabrata, krusei, tropicalis, parapsilosis and Candida famata respectively. Also findings suggested that fluconazole was able to inhibit the growth of candida species at an expanded range of concentration between 4-128 microgeram per milliliter.As well antifungal activity of Fluconazole with silver nanoparticles was increased in comparision with using Fluconazole alone. For the prevention of recurrent cases and to stabilish correct diagnosis it is essential to carry out sensitivity and diagnostic tests in laboratory and also administration of silver nanoparticle in combination with fluconazole in drug formulation for topical uses in treatment of chronic vaginal candidiasis and inhibition of recurrent cases, can useful

[ comparison of the bayer acryl and acropars acryl effect on the adhesion of candida albicans]

Candidiasis is one of the most common fungal infections in oral cavity. About 85% of this infection is caused by Candida albicans a flora of mouth. Since one of the problems in denture users is it's contamination with C.albicans and discoloration, and lack of information about adhesion of C.albicans to different resins, we designed this study to compare the effect of two types of acrylic resin on adhesion of C.albicans. This was an experimental study within that 36 samples from Bayer and Acropars acrylic resin were added in tubes containing suspension of 1-106 [CFU/ml] of C.albicans. Then tubes were incubated at 37? c for 40 and 120 minutes. After incubation, the samples were transferred in 1ml of sterile saline and after resuspension by shaking a known quantity of each was inoculated on sabouraud dextrose agar and incubated at 37? c for 48 hours and after that, formed colonies were counted and were analyzed by Mann-u-Whitney test. These two acrylic resins did not reveal statistical significant differences in two 40? c and 120? c different lengths of the time from the point of adhesion. Also Bayer acrylic resin did not show any significant differences from the point of adhesion in to different lengths of times. But dissimilarly was significant for Acropars acrylic resin which indicated adhesion with the passage of the time. [p<0.1] These two Acrylic resins do not have any priority to each other from the view point of non-adhesion to Candida albicans, but Bayer acryl is more appropriate particularly for those who are with weak hygiene and are more susceptible to fungal infections. Surface roughness of acrylic resins and their components, characteristic and surface factors have essential role in adhesion of C.albicans