RESUMO
Background: Monolayer electrospun scaffolds have already been used in bone tissue engineering due to their high surface-tovolume ratio, interconnectivity, similarity to natural bone extracellular matrix [ECM], and simple production
Objectives: The aim of this study was to evaluate the dynamic culture effect on osteogenic differentiation and mineralizationi into a compact cellular multilayer nHA-PCL electrospun construct. The dynamic culture was compared with static culture
Materials and Methods: The calcium content, alkaline phosphatase [ALP] activity and cell viability were investigated on days 3 and 7
Results: When the dynamic culture compared to static culture, the mineralization and ALP activity were increased in dynamic culture. After 7 days, calcium contents were 41.24 and 20.44 micro g.[cm[3]][-1], and also normalized ALP activity were 0.32 and 0.19 U.mg[-1] in dynamic and static culture, respectively. Despite decreasing the cell viability until day 7, the scanning electron microscopy [SEM] results showed that, due to higher mineralization, a larger area of the construct was covered with calcium deposition in dynamic culture
Conclusions: The dynamic flow could improve ALP activity and mineralization into the compact cellular multilayer construct cultured in the perfusion bioreactor after 7 days. Fluid flow of media helped to facilitate the nutrients transportation into the construct and created uniform cellular construct with high mineralization. This construct can be applied for bone tissue engineering