RESUMO
The extension of platelet storage from five to eight days was based on the functional quality and bacterial safety. It was the aim of the current study to implement a testing strategy for extended platelet shelf life up to 8 days with optimal achievement of patient safety and adequacy of treatment. Twenty random-donor platelet concentrates RDPCs were prepared under routine conditions by platelet-rich plasma [PRP] protocol then stored at 22°C under Constant agitation for 8 days. The storage changes were assessed on days 1, 5 and 8. for morphology [platelet indices and swirling], metabolic function [p1-I and glucose], cell integrity [lactate dehydrogenase "LDH"], and activation markers [flow Cytometrie analysis of CD62 and CDG3]. Correlation analysis, between the studied parameters at previous time-points storage, was performed. Microbiological culture was carried out on days 1 and 8 using automated microbial detection BACTEC system. A significant decrease in platelet counts during storage, accompanied by significant increase in mean platelet volume [MPV], was noticed. Changes in pH, glucose, and swirling were within the acceptable range. LDH levels were significantly increased on days 5 and 8. Although a gradual increase of CD62 and CD63 surface expression was seen with increasing storage time, it was not statistically significant except at day 1 for both markers and day 8 for CD62 only. A correlation between pH and glucose levels at days 5 and 8 was observed and between pH and CD62 and CD63 at days 8 and 5 respectively. All the 20 PC units were culture negative. We concluded that, the in vitro quality of the stored platelets was maintained until day 8 namely platelet Count, indices and swirling phenomena. In addition, pH, glucose remained within the acceptable range, while recorded LDH levels were considerably increased. Platelets were found to be activated at day 1, likely as a consequence of manipulation during collection and processing, but are not further progressively activated with increasing storage time. BACTEC system is suitable as rapid screening bacterial detection device for PCs