Study of trichomoniasis among egyptian male patients

In the present study, Trichomonas vaginalis was diagnosed in 28.8% male patients with urethral discharge and in 8.2% suffering from impotence and infertility. Diagnosis was based on examination of urethral discharge, urine, semen and prostatic massage fluid by wet mount, stained films and culture inoculation. Diamond's culture proved to be the method of choice

immunological aspects of cutaneous leishmaniasis in Egypt

In patients with cutaneous leishmaniasis, B and T-lymphocytes subpopulations [CD3[+], CD4[+] and CDB[+] were estimated. In cases and with different clinical forms diagnosis was made by stained smears, culture on Schneider's medium with 25% foetal calf serum. The results revealed that CD4[+] were significantly increased in early infection [less than 6 weeks]. No significant changes were found in single lesion in contrast with multiple lesions [more than 6 weeks] where CD4[+] significantly decreased than controls. CD8[+] were increased with the both the number and duration of the lesions. Follow up of cases till spontaneous clinical and parasitological cure showed no significant difference in comparison to controls. It was concluded that T-lymphocyte subpopulations through their cytokine mediators may play an important role in the mechanism of healing in cutaneous leishmaniasis

Prevalence of toxoplasma gondii and cytomegalovirus antibodies in patients with chronic renal failure

This study aimed to determine the prevalence of Toxoplasma gondii and cytomegalovirus [CMV] antibodies in patients with chronic renal failure [CRF] with and without hemodialysis. To achieve this aim, 60 patients with CRF, in addition to 20 healthy controls were investigated. Patients were classified into 3 groups: Group I comprised 20 patients with CRF who have not any hemodialysis sessions, under conservative treatment, group II comprised 20 patients with CRF due to primary renal diseases already on hemodialysis and group III comprised 20 patients with renal failure secondary to collagen disease or accompanied by liver disease undergoing hemodialysis. Out of 60 patients, Toxoplasma antibodies were detected in 38.3% and in 15% of the normal controls with a statistical significant difference. High percentage of positivity for Toxoplasma and CMV antibodies in patients with CRF undergoing hemodialysis was noticed, which was also correlated to the number of the dialysis sessions. So, it can be concluded that CRF patients undergoing hemodialysis should be screened for toxoplasma and cytomegalovirus before dialysis to prevent the dissemination of these infections through dialysis procedure

On employing leishmania promastigotes for systemic lupus erythematosus diagnosis

Based on having definite double stranded DNA determinants in the kinetoplast, it was attempted to use Leishmania promastigotes as an IFAT substrate for the detection of serum anti ds-DNA autoantibodies which are the prominent diagnostic marker of SLE. Serological inspection of different Leishmania preparations revealed that Leishmania is not analogous to Crithidia lucilia as an appropriate ds-DNA substrate. It seems likely that the species-specific molecular characters of the kinetoplast-DNA are influencing the reaction. The issue is an evidence of the distinct differentiation of the K-DNA antigenic determinants in haemoflagellates