Isolation and Characterization of Microbes of Different Kinds of Street Food and Determination of Antibiotic Susceptibility of the Isolates

Aim: Improper personal hygiene can facilitate the transmission of the pathogenic microorganisms found in environment and on people抯 hands via food to humans. The present study was undertaken to investigate the microbiological quality of different street food and determining the antibiotic susceptibility of the isolated microorganisms. Study Design: Collection of food samples for isolation of Pathogenic Microorganisms, to identify them by using Biochemical test, molecular test (16sr RNA typing), Antibiotic susceptibility was done by using different antibiotics against the isolates. Place and Duration of Study: Food samples were collected from street vendors of Sainkpuri area, work was done from December 2020 to April 2021 at Microbiology Department, St. Francis College for Women, Hyderabad. Methodology: Five samples of street food were collected in sterilized bottles, tested for the presence of microorganisms by following standard microbiological method used for isolation of microorganisms. The organisms were identified by carrying out various biochemical test according to Bergey抯 Manual of Systematic Bacteriology. The Molecular characterization was done based on 16sr RNA typing. Determining the sensitivity of the isolates against different Antibiotics by employing Kirby Bauer technique. Results: The organisms isolated from Manchuria and Ragada samples: Lactobacillus delbrueckii; Lactobacillus casei, Klebsiella pneumonia, Enterococcus faecalis and Vibrio spp respectively; Samosa and bonda: Staphylococcus aureus, Lactobacillus delbrueckii and Pantoea dispersa; Pani puri water: Staphylococcus aureus and Providencia vermicola. Antibiotic susceptibility tests showed that most of the isolated microorganisms were sensitive to Ciproflaxin. Conclusion: Hence the quality of street food is found to be low due to following factor: lack hygiene conditions in the food preparation, ingredients may also affect quality of the food, and presence of air borne microorganisms in the surrounding areas of service points.

Prevalence and Risk Correlates of Hypertension among Adults in Rural Field Practice Area of Tertiary Care Medical College at South India.

Background: In India, lifestyle diseases were considered in the urban setting; so the medical fraternity has concentrated more on the urban people. With epidemiological transition, a dramatic reduction in physical exercise has been observed in the rural areas with an increase in the stresses of life. According to World Health Report 2002, cardiovascular diseases will be the largest cause of death and disability by 2020 in India; the contributing factors are increasing hypertension, dyslipidemia, diabetes, overweight or obesity, physical inactivity, and using tobacco. Our study aims to determine prevalence of hypertension in an adult rural community and to identify the risk factors of Hypertension among adults in Rural Field Practice Area Singanodi of Navodaya Medical College, Raichur, Karnataka. Methods: The present study is a cross sectional study, carried out in village Singanodi, which is designated as rural field practice area of Community Medicine Department, Navodaya Medical College. It has population coverage of 14,200.The study population include all subjects who were aged 30 years and above. The present study was a population based study. Results: Out of total population, 240 (37.3%) were in age group of 30-39 years, 222 (34.5%) were in age group of 40-49 years. Males and females were 386 (60.03%) and 257 (39.97%) respectively. Our study revealed that hypertension was significantly associated with body mass index, maximum in obese patients when compared to normal and underweight (p<0.0001) There is significant association between smoking and hypertension 77 (29.9%). (p<0.015) There is significant association with physical activity; that is sedentary people were more prone for getting hypertension when compared to heavy worker. However, salt intake > 6 grams/day and family history of hypertension were found to be significantly associated. (p=0.009; p=0.003 respectively) The prevalence of hypertension among subjects on vegetarian diet vs. mixed diet was 23.9% vs. 22.2%, respectively but the difference was not statistically significant. (p= 0.09) Conclusion: From our study we conclude that there is a high prevalence of hypertension even in rural area and advocate to flourish awareness regarding modifiable risk factors of hypertension among the people.

Uterine low grade endometrial stromal sarcoma: a case report with review of literature.

Endometrial stromal sarcomas are rare uterine tumours usually seen in perimenopausal females. We report here a case of low grade endometrial stromal sarcoma in a 45 year female with a clinical diagnosis of fibroid uterus and final histological diagnosis of low grade endometrial stromal sarcoma.