Myosin-V is present in synaptosomes from rat cerebral cortex

the subcellular localization in brain of an unconventional, calmodulin-binding myosin (myosin-V) found in neurons, astrocytes and other secretory cells of vertebrates has been investigated by probing Western blots of synaptic fractions from rat cerebral cortex with affinity-purified polyclonal antibodies against myosin-V. Myosin-V was detected in intact synaptosomes and in lysed synaptosomes associated with a particulate fration. Our data suggest a role for brain myosin-V in membrane-cytoskeleton function in the synaptic region