Expression and correlation of P73 and PS3 proteins in esophagus squamous cell carcinoma / 国际肿瘤学杂志

Objective To investigate the expression and correlation of P73 and PS3 proteins in esophagus squamous cell carcinoma. Methods The immunohistochemistry staining assay was conducted to detect the expression of PS3 and P73 proteins in 46 cases with squamous cell carcinoma of esophagus and 30 normal mucosa cases. The protein expressions and clinic pathological observation and the correlation between two proteins were statistically analyzed. Results The expression of P53 and P73 proteins showed differences between esophageal cancer tiksue and normal mucosa ( P = 0.007,0.008) . The expression of P53 appeared to be correlated with lymph node metastasis(P = 0.047) and gender(P =0.028),but not correlated with age, the depth of invasion , differentiation of cancer tissue, gross observation and position of the tumor( P > 0.05 ). The expression of P73 appeared to be correlated with differentiation of cancer tissue and lymph node metastasis ( P = 0. 023, 0.035), but not correlated with age, gender, the depth of invasion,gross observation and position of the tumor (P >0.05). P73 expression was positively corelated with P53 expression in squamous cell carcinoma of the esophagus ( r = 0.359 ,P = 0.014 ). Conclusion Both P73 and P53 proteins are associated with the occurrence of squamous cell carcinoma of the esophagus and participate in the development of esophagus squamous cell carcinoma.

Determination and clinical significance of amino acid transmitters in plasma of the patients with ischemic vascular disorders / 中华检验医学杂志

Objective To observe the determination and clinical significance of amino acid transmitters in plasmas of the patients with ischemic vascular disorders.Methods Excitatory and inhibitory amino acids in plasmas of 57 old peoples with ischemic vascular disorders and 33 normal old peoples were determined with high performance liquid chromatography(HPLC).Results Significant increases were found in plasma concentration of glutamate(26.48%)、aspartate(39.70%), ?-aminobutyric acid(GABA,74.57%), glycine(36.79%) and alanine(21.33%) within fist three days after cerebral ischemia compared to normal controls. The content of glutamate and aspartate began to increase significantly in 24 hours after ischemia. The plasma levels of glutamate, aspartate and GABA have returned to normal in recovery patients.Conclusions These results showed that the concentration changes of excitatory and inhibitory amino acids in plasmas may suggest the early happening and severity of cerebral ischemia.

The diagnostic value of secretory phospholipase A_2 and lysophosphatic acid for ovarian cancer / 中华检验医学杂志

Objective To explore the diagnostic value of secretory phospholipase A2 (sPLA2) and lysophosphatic acid (LPA) for ovarian cancer and evaluate the correlation of sPLA2 activity and LPA level to the prognosis in the patients with ovarian cancer.Methods ~3H oleic acid labeled E. coli membrane and spectrophotometry were used to detect the original and recurrent ovarian cancer. The motive observation between sPLA2 activity and LPA level was done.At the same time, The sensitivity and specificity for the diagnosis of ovarian cancer were analyzed.Results Comparing the original ovarian cancer group with the ovarian benign tumor group, the difference between sPLA2 activity (24.47 ?5.64 U/L,4.94 ?1.86 U/L) and LPA levelc(6.35 ?2.32 ?mol/L,1.92 ?0.75 ?mol/L) were significantly (P