RESUMO
This study was carried out to see the response of ovariectomy in adult female rats as determined by rat Luteinising Hormone [rLH] Radioimrnunoassy [RIA]. Animals were divided in two groups. Group I [control, n=6] were sham ovariectomised and group II [experimental, n=4] were ovariectomised. Next day at 1400 hours rats were sacrificed and their plasma was collected for rLH, RIA. It was observed that there was highly significant increase in plasma rLH [P<0.001] in experimental group as compared to controls. Also the increase in the weights of hypothalamus and anterior pituitary of experimental group was highly significant [P<0.001] as compared to controls. This shows that this rise in plasma rLH and weights of hypothalamus and anterior pituitary was due to loss of negative feed back effect of ovarian steroids