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Chinese Journal of Virology ; (6): 370-378, 2015.
Artigo em Chinês | WPRIM | ID: wpr-296274

RESUMO

To study the complete genomic sequence, genomic characteristics, and genetic variation of the bovine papillomavirus 2 genotype (BPV-2) Aks-01 strain at the molecular level, genotyping of this strain from the skin samples of cows in southern Xinjiang (China) was first detected by the polymerase chain reaction with FAP59/FAP64 primers. Based on the complete genome of the BPV-2 reference strain, specific primers and sequencing primers were designed, and the complete genome of the Aks-01 strain amplified and sequenced. Sequence analyses showed that genotyping of the Aks-01 strain belonged to BPV-2. The Aks-01 strain had the structural characteristics of BPV-2. The 7944-bp full-length genomic sequence of the Aks-01 strain was compiled using DNAStar™. The sequence of the Aks-01 strain had 98% similarity to the reference strain from GenBank. The Aks-01 strain was most closely related to BPV-1 and BPV-13. BPV-2, BPV-1 and BPV-13 were grouped within the genus Deltapapillomavirus. The Aks-01 strain is the first BPV-2 strain reported in southern Xinjiang.


Assuntos
Animais , Bovinos , Feminino , Sequência de Aminoácidos , Sequência de Bases , Papillomavirus Bovino 1 , Genética , China , Evolução Molecular , Genoma Viral , Genética , Genômica , Genótipo , Dados de Sequência Molecular , Proteínas Oncogênicas Virais , Química , Genética , Metabolismo , Filogenia , Análise de Sequência de DNA , Pele , Virologia
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