Generation of human monoclonal antibody Fab fragment to Zika Virus by Phage Display Technology / 中华实验和临床病毒学杂志

Objective@#In this study, phage display technology was used to construct the human anti-Zika virus(ZIKV), phage antibody library and to obtain and express the monoclonal antibody. The aim was to master the preparation and expression of human phage antibody library screening method for highly specific antibodies.@*Methods@#The whole blood samples of Zika patients were collected and the lymphocytes were isolated. The RT-PCR method was used to amplify the antibody light chain and heavy chain Fab gene from lymphocyte Ig mRNA. The pComb3H system was used to construct the gene with genetic diversity Preparation of human anti-ZIKV phage antibody library. The purified antibody library was screened by using the purified ZIKV and the obtained ZIKV E protein antigen.@*Results@#The monoclonal antibody Fab fragment gene was successfully obtained for the ZIKV E protein antigen. The gene can be efficiently expressed in Escherichia coli.@*Conclusions@#According to the sequence analysis, this study showed that the monoclonal antibody was a new human genetically engineered antibody against ZIKV, which laid the foundation for the early diagnosis of ZIKV, and obtain a specific monoclonal antibody to ZIKV for human treatment of ZIKV infection.

Study of G-6-PD Deficiency of Blood Donors in Suining Region, Sichuan Province and Influence of Being Transfused with Blood from Donors Eating Broad Beans on Children with Favism / 中国输血杂志

A study of G-6-PD state in red cells of 2483 blood donors was performed using the improved blue tetrazolium method ,indicating that 8.3% of the donors studied were in G-6-PD deficiency and 2. 1% of the donors studied were in severe G-6-PD deficiency. The files of blood donor G-6-PD state were established to prevent the children with favism from being transfused with G-6-PD deficient blood, thereby the safety of blood transfusion could be enhanced. The impact of being transfused with the blood extracted from the donors at different time (2~4 hr. 12-24hr. and 2-3 days )after eating broad beans on the children with favism was observed. There was no obvious difference in the improvement of clinical symptoms,restoration of hemoglobin in peripheral blood and change of G-6-PD activity between the tested and control groups. There were no found the cases of rehemolytic reaction in either group,indicating that being transfused with the blood from donors eating the broad beans had no evident adverse influence on the children with favism during the "refractory period" following the acute hemolysis.