RESUMO
Microalgae produce a wide range of compounds including pigments, protein, starch, and lipids, which havebeen extensively used for various applications. In the current scenario, microalgal lipids are considered as apromising source for the production of next-generation bioenergy, and a huge productivity is needed to meet thedemand. Thus, to increase the production of biomass and lipid content, physical conditions play an importantrole and necessary to be optimized. The present study made such an attempt to optimize the physical factors forthe growth and production of lipid from Nannochloropsis gaditana. The study aimed to determine the effect ofphysical parameters such as pH, temperature, light intensity, and salinity. The results showed that the maximumgrowth rate of the N. gaditana was noticed in the salinity of 25 ppt, pH of 8, temperature of 25°C, and 2,000 luxof light intensity. The highest lipid content of the N. gaditana was noticed in the salinity of 30 ppt, pH of 8, andtemperature of 30°C with 2,000 lux light intensity. After optimization, above 40% of lipid yield was obtained,and it can be effectively utilized in bioenergy production.