RESUMO
The functional characterisation of thousands of
Assuntos
Vetores Genéticos/genética , Trypanosoma cruzi/genética , Cromatografia de Afinidade , Clonagem Molecular , Etiquetas de Sequências Expressas/metabolismo , Expressão Gênica/genética , PlasmídeosRESUMO
Calpains are calcium-dependent cysteine proteinases found in all living organisms and are involved in diverse cellular processes. Calpain-like proteins have been reported after in silico analysis of the Tritryps genome and are believed to play important roles in cell functions of trypanosomatids. We describe the characterization of a member of this family, which is differentially expressed during the life-cycle of Trypanosoma cruzi.