Heteroantagonismo entre estirpes de Enterobacter agglomerans isoladas de urubu (Coragyps atratus) como produtoras e Pseudomonas aeruginosa como reveladoras / Evaluation of the heteroantagonism between Enterobacter agglomerans strains isolated from vulture (Coragyps atratus) for production and Pseudomonas aeruginosa as developed

Avaliou-se o heteroantagonismo entre Enterobacter agglomerans isolada do trato gastrintestinal de urubu (Coragyps atratus) e Pseudomonas aeruginosa isoladas do ambiente hospitalar. Foram utilizados o método de sobrecamada ou lento e a técnica direta ou de poços. Pelo método da sobrecamada, de 196 testes realizados para pesquisa da atividade antagonista, foi detectada a presença de halos de inibição relacionados ao fenômeno de heteroantagonismo em 118 deles (60,2 por cento). Pelo método de poços, obtiveram-se resultados semelhantes. As sete amostras de E. agglomerans foram capazes de realizar heteroantagonismo nas condições testadas, que foram detectados pela formação de halos claros de inibição. O extrato de levedura adicionado a 1 por cento no meio de cultura foi um suplemento adequado para a demonstração do antagonismo.

The heteroantagonism between Enterobacter agglomerans, isolated from the gastrointestinal tract of American vulture Coragyps atratus, and Pseudomonas aeruginosa isolated from a hospital environment was evaluated. The slow (layer) and the wells (direct) techniques were tested, using agar and soy tryptone broth pH 7.3 at 37ºC. Through the slow method from 196 tests, inhibition growth halos, related heteroantagonism phenomenon observed in 118, corresponding to 60.2 percent positive results. Equivalent positive results were detected using wells (direct) methodology. The seven samples of E. agglomerans tested were capable of revealing heteroantagonism in the experimental conditions; antagonism reveled by the presence of a clear growth inhibition halo. The added 1 percent yeast extract to media was adequate for revealing antagonisms best.

Organization and structure of an Onchocerca ß-tubulin gene

In filarial worms, as in other eukaryotes, microtubules are essential multifunctional components. The major protein of microtubules is tubulin, a heterodimer of two distinct polypeptides, Ó e ß.Tubulin is particulary important in helminthic parasites as a target for anthelminthic benzimidazoles, wich bind to it and inhibit microtubule assembly. Two genomic Onchocerca gibsoni libraries were constructed in NM1149(EcoRi and HindIII). Three clones accounted for the entire gene: one from the EcoRi library (using a Plasmodium falciparum probe) containing the central part of the gene, and two from the HindIII library (using as probes PCR amplified fragments from the ends of the EcoRI clone) which, respectively, contained the 5'- and 3' -ends of the gene. The sequencing procedure for the EcoRI clone relied on the construction of a double-digested DraI/HindIII shotgun library. A number of recombinants were sequenced and aligned with each other for comparison. The sequencing of the overlapping 5' - and 3'-end clones was done by ...