Frequency of the DI*A, DI*B and Band 3 Memphis polymorphism among distinct groups in Brazil

ABSTRACT Introduction: The Band 3 is a red blood cell protein that carries the Dia and Dib antigens from the Diego blood system. The SLC4A1 gene encodes Band 3; Band 3 Memphis is a polymorphism of normal Band 3 and has two variants, but only the variant II carries the Dia antigen. Objectives: Describe the frequencies of the DI*A and DI*B alleles and the Band 3 Memphis among blood donors, sickle cell disease (SCD) patients and Amazonian Indians. Methods: A total of 427 blood samples were collected and separated into three groups: 206 unrelated blood donors, 90 patients with SCD and 131 Amazonian Indians. We performed DI*A/B, normal Band 3 and Band 3 Memphis genotyping, using the Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP). Results: The frequency of the DI*A/DI*A genotype was 0.5% in blood donors and it was not found in other groups. The frequency of the DI*A/DI*B was higher in Amazonian Indians (33.6%) and the frequency of the DI*B/DI*B was highest in blood donors (92.2%). All 105 individuals tested were positive for the presence of normal Band 3 and of these individuals, only 5/105 (4.8%) presented the Band 3 Memphis mutation. Conclusion: We observed a higher frequency of the DI*B allele in blood donors and a low frequency of the DI*A/DI*A genotype in all groups studied. The Band 3 Memphis was found in a higher frequency in the blood donor group. Our findings highlight the importance of analyzing different population groups to gain a better understanding of the genetic association of blood group antigens.

Sexualidade de mulheres submetidas à mastectomia: identificação das fases afetadas no ciclo da resposta sexual / Sexuality of women submitted to mastectomy: identification of phases affected in the sexual response cycle / Sexualidad de mujeres submetidas a mastectomía: identificación de las fases afectadas en el ciclo de la respuesta sexual

Objetivo: analisar a sexualidade das mulheres mastectomizadas, identificando quais as fases do ciclo da resposta sexual são afetadas e quais as disfunções desenvolvidas. Método: foi utilizando um questionário com dados socioeconômicos e o Questionário Quociente Sexual - versão feminina, trata-se de uma pesquisa descritiva de cunho quantitativo, com mulheres mastectomizadas em atendimento no ambulatório de Cuidado oncológicos. Resultado: o desempenho sexual da população estudada apresentou-se heterogêneo, onde 50% apresentou padrão sexual satisfatório e 50% algum tipo de comprometimento na sexualidade, e dentro deste grupo, incluem-se as casadas, com menos de 49 anos e com grau de instrução de nível fundamental. Conclusão: diante do exposto, os profissionais de saúde em geral devem estar atentos para a ocorrência dessas modificações na vida dessa mulher, diante do todo o processo, desde diagnóstico, no tratamento e readaptação, para dessa forma contribuir para o cuidado integral

Objective: analyze the sexuality of women with mastectomies, identifying the phases of the cycle of sexual response are affected and which developed dysfunctions. Method: using a questionnaire with socioeconomic data and Questionnaire Sexual Quotient - female version. This is a descriptive quantitative nature with mastectomies women in the outpatient clinic of oncological. Result: Sexual performance of the studied population presented a heterogeneous, where 50% showed satisfactory sexual standard and 50% some kind of commitment in sexuality, and within this group, married include, under 49 years old and with education level of fundamental level. Conclusion: given the above, the general health professionals should be alert to the occurrence of these changes in the life of this woman, before the whole process, from diagnosis, treatment and rehabilitation, to thereby contribute to the comprehensive care

Objetivo: analizar la sexualidad de las mujeres mastectomizadas, la identificación de las fases del ciclo de respuesta sexual están afectadas y las disfunciones desarrollado. Metodo: utilizando un cuestionario con datos socioeconómicos y Cuestionario Cociente Sexual - versión femenina. Se trata de un carácter cuantitativo descriptivo con mastectomías mujeres en el ambulatorio del oncologia. Resultados: el rendimiento sexual de la población estudiada presentó un grupo heterogéneo, donde el 50% mostró norma sexual satisfactoria y el 50% algún tipo de compromiso en la sexualidad, y dentro de este grupo, se casó incluir, en virtud de 49 años de edad y con un nivel de educación de las nivel fundamental. Conclusiones: teniendo en cuenta lo anterior, los profesionales de la salud en general deben estar alerta ante la aparición de estos cambios en la vida de esta mujer, antes de que todo el proceso, desde el diagnóstico, tratamiento y rehabilitación, para contribuir así a la atención integral