In vitro antimicrobial activity of iris pseudacorus and urtica dioica

In this study, the effects of antibacterial activity of Urtica dioica and Iris pseudacorus essential oils, native plant northern of Iran, were investigated for some selected bacteria. The influence of essential oils was tested by the using of disk diffusion and micro-broth dilution methods against standard strains of the picked out bacteria. Gas Chromatography/Mass Spectroscopy [GC/MS] analysis, bioactivity determination, Minimum inhibitory concentrations [MIC] and minimum bactericidal concentration [MBC] of essential oils were utilized for this goal. This study showed that, Inhibition zone diameter varied from 11 to 19 mm and 9 to 17 mm for Urtica dioica and Iris pseudacorus respectively. In contrast, this figure fluctuated from 19 to 28 mm and 7 to 17 mm for gentamicin and ampicillin separately. By the application of micro-broth dilution technique, MICs for 1% essential oils were 1.8-7.5 micro g/ml and 3.75-15 micro g/ml for, Urtica dioica and Iris pseudacorus against gram-positive and gram-negative bacteria individually. Furthermore, the MBCs of herbal essences were 1.8-15 micro g/ml for, Urtica dioica and 15-30 micro g/ml for Iris. The application of essential oils for the bio-control of diseases, as a novel emerging alternative to antimicrobial treatments, lead to safer and more environmental management for infective diseases

Evaluation of antifungal activity of methanol extract of acacia [anagalis arvensis] leaves and nystatin against candida albicans in vitro

Anagalis is the name of Scarlet Pimpernel, with about 20 to 25 species of flowering plants in the family Myrsinaceae. The common variety [A. arvensis] is a low-growing annual plant found in Europe, Asia and North America. In this Investigation, the inhibitory effects of methanol extract of Anagalis arvensis leaves and drug nystatin on clinical isolates and standard strain of Candida albicans [14053 PTCC] isolated from patients with vulvovaginal candidiasis were studied. In the laboratory study of 45 clinical isolates of Candida albicans was used and inhibitory effects of the extract were evaluated using by serial dilution Method. Chemical compounds of A. arvensis extract including alkaloids, glycosides, phenolic compounds, Terpenoids and amino acids were identified. The results showed that the minimum inhibitory concentration [MIC] nystatin against clinical isolates and standard strain of Candida albicans were similar and drug nystatin revealed the better MIC against standard strain and clinical isolates of C. albicans. Methanol extract of A. arvensis were inhibitory on standard strain and clinical isolates of C. albicans. The minimum inhibitory concentration of extract in comparison with the drug nystatin was less while the composition of the growth inhibitory concentration was greater than drug solely. This study indicated that the antimicrobial activity of this plant extract was considerable compared to drug nystatin and it demonstrate a basis for further research to isolate the active components in a variety of microbial strains