RESUMO
Background: Expansion and differentiation of stem cells relies on the soluble materials as well as the physical conditions of their microenvironment. Several methods have been studied in attempt to enhance the growth and differentiation rates of different adult stem cells extracted from different sources
Objectives: The purpose was to improve the three-dimensional [3D] culture condition of the semi-permeable polymeric beads for encapsulation of the human adipose-derived mesenchymal stem cells [hADSCs] by modifying the ratio of the alginate-gelatin composition
Materials and Methods: Following isolation and characterization of hADSCs by flow cytometry and their functional differentiation, encapsulation in the alginate and alginate/gelatin compositions were performed. Moreover, the stability, swelling, size frequency, growth kinetics, and cytotoxicity of the beads were measured to meet proper condition in the designed experimental and control culture conditions. Finally, the growth rates of the cells in different experimental groups and control were measured and analyzed statistically
Results: Viability decreased in 2 and 3 percent alginate once compared to 1% alginate in beads [p
Conclusions: These findings suggested for the first time that the composite of alginate/gelatin beads with the ratio of 50:50 might provide a suitable culture condition for the encapsulation and in vitro expansion of the hADSCs