RESUMO
Current scolicidal agents, which have been used for inactivation of protoscoleces during hydatid cyst surgery are associated with adverse side effects. This study aims to evaluate the in vitro scolicidal effects of Nectaroscordum tripedale L. leave extract against protoscoleces of hydatid cysts and its acute toxicity in mice model. Various concentrations of the extract [12.5-100 mg/mL] were used for 5 to 30 min. Viability of protoscoleces was confirmed using eosin exclusion test [0.1% eosin staining]. In addition, the acute toxicity of N. tripedale extract was determined for 2 days in mice model. The results showed that the N. tripedale extract at the concentration of 100 mg/mL after 5 min of exposure killed 100% protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 10 min of exposure to concentration of 50 mg/mL was 100%. The LD50 values of intraperitoneal injection of the N. tripedale extract was 3.36 g/kg body wt. and the maximum nonfatal doses were 2.98 g/kg body wt. The results showed the potential of N. tripedale extract as a natural source for the production of new scolicidal agent for use in hydatid cyst surgery
RESUMO
Objective: To determine the chemical composition, antioxidant activity and antibacterial properties of essential oils of the aerial parts of Salvia sclareoides (S. sclareoides). Methods: The essential oil of the areal parts of S. sclareoides was obtained by using hydrodistillation method and the composition of the volatile components analyzed by gas chromatography method coupled with mass spectrometry detector. The antimicrobial capacity of the essential oil of S. sclareoides was investigated by microdilution technique. The antioxidant activities were determined employing inhibition of 2,2-diphenyl-1-picrylhydrazyl hydrate radical method. Results: Mass spectra were searched against mass spectrometry databases and sixty components were recognized. Non-terpenoids (41.6%) and sesquiterpenes (39.7%) were determined as the main components of the essential oil. The main identified components were, linalool (27.6%), trans-caryophyllene (16.6%), beta.-trans-ocimene (11.831%), germacrene-D (10%), bicyclogermacrene (3.3%) and caryophyllene oxide (2.8%). Two monoterpens (13.2%) and three oxygenated sesquiterpenes (5.5%) were also obtained from the essential oil of the S. sclareoides. Conclusions: Results indicated that essential oil of S. sclareoides includes rather higher proportions of non-terpenoid and sesquiterpenes compounds with good antioxidant and antibacterial properties.
RESUMO
OBJECTIVE@#To determine the chemical composition, antioxidant activity and antibacterial properties of essential oils of the aerial parts of Salvia sclareoides (S. sclareoides).@*METHODS@#The essential oil of the areal parts of S. sclareoides was obtained by using hydrodistillation method and the composition of the volatile components analyzed by gas chromatography method coupled with mass spectrometry detector. The antimicrobial capacity of the essential oil of S. sclareoides was investigated by microdilution technique. The antioxidant activities were determined employing inhibition of 2,2-diphenyl-1-picrylhydrazyl hydrate radical method.@*RESULTS@#Mass spectra were searched against mass spectrometry databases and sixty components were recognized. Non-terpenoids (41.6%) and sesquiterpenes (39.7%) were determined as the main components of the essential oil. The main identified components were, linalool (27.6%), trans-caryophyllene (16.6%), beta.-trans-ocimene (11.831%), germacrene-D (10%), bicyclogermacrene (3.3%) and caryophyllene oxide (2.8%). Two monoterpens (13.2%) and three oxygenated sesquiterpenes (5.5%) were also obtained from the essential oil of the S. sclareoides@*CONCLUSIONS@#Results indicated that essential oil of S. sclareoides includes rather higher proportions of non-terpenoid and sesquiterpenes compounds with good antioxidant and antibacterial properties.