RESUMO
ABSTRACT Objective: To evaluate the antibacterial effect of electrolytically generated hypochlorous acid on Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis. Material and Methods: In this in vitro experiment, the effect of hypochlorous acid (HOCl) on the strains S. gordonii, F. nucleatum, and P. gingivalis was evaluated using 4% sodium hypochlorite, 0.12% chlorhexidine, and distilled water as controls. The four groups were placed on each plate, and each group was replicated five times. The agar diffusion method by zones measurement was used. The data were processed with SPSS using the Kruskal-Wallis test and multiple comparison tests. Results: Hypochlorous acid showed an average inhibition halo of 9.28 mm on S. gordonii. As expected with distilled water, no zone of inhibition was noted for any of the bacteria, nor were zones of inhibition observed with HOCl for F. nucleatum and P. gingivalis. Conclusion: Hypochlorous acid showed antimicrobial properties against only S. gordonii and was less effective than 4% sodium hypochlorite and 0.12% chlorhexidine, although no significant differences were found between the latter.
Assuntos
Hipoclorito de Sódio , Antibacterianos/imunologia , Doenças Periapicais , Análise de Variância , Estatísticas não ParamétricasRESUMO
Abstract Objective: To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitro. Material and Methods: Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test. Results: The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii (p=0.000), F. nucleatum (p=0.000), and P. gingivalis (p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction. Conclusion: The methanol fraction of P. guajava had an antibacterial effect on S. gordonii and P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion.
Assuntos
Periodontite/microbiologia , Biofilmes , Psidium/química , Streptococcus gordonii/patogenicidade , Antibacterianos/farmacologia , Infecções Estreptocócicas , Técnicas In Vitro , Análise de VariânciaRESUMO
La intususcepción intestinal es una patología infrecuente en el adulto causante de 1%-5% de obstrucciones intestinales, se asocia con frecuencia a patologías de origen neoplásico o alteraciones estructurales del tracto gastrointestinal, sin embargo hasta en el 10% de los casos es idiopática. En la mayoría de los casos el diagnóstico se realiza después de la intervención quirúrgica, pero con el desarrollo de técnicas de imagen, en la actualidad, se puede llegar a la presunción diagnóstica previa intervención. Se presenta un caso clínico en el cual el diagnóstico se realiza de manera preoperatoria por medio de tomografía computarizada y la revisión de literatura correspondiente.
Intestinal intussusception is an uncommon condition in adults which causes 1% to 5% of intestinal obstructions. Although it is frequently associated with neoplastic pathologies or structural abnormalities of the gastrointestinal tract, up to 10% of cases are idiopathic. In most cases the diagnosis is made after surgery, but with the development of imaging techniques, a diagnosis is possible prior to intervention. This article presents a case in which the diagnosis was made preoperatively by CT scan and presents a review of the relevant literature.
Assuntos
Humanos , Feminino , Adulto , Adulto , Obstrução Intestinal , IntussuscepçãoRESUMO
El cáncer de cuello uterino es una alteración neoplásica de crecimiento lento, descontrolado y persistente del epitelio exocervical y endocervical, intraepitelial, luego invasivo. Es la primera causa de muerte por cáncer en la mujer. Para evaluar la cobertura alcanzada en la aplicación del Programa de Cáncer de Cuello Uterino en cuanto a pesquisas en Nueva Esparta durante el trienio 2000-2002. Se utilizaron datos de las citologías realizadas por la dirección del programa, encuestas y evaluación de los ambulatorios. Educación para la salud 81,39 por ciento, es adquirida en los ambulatorios, medios de comunicación 33,72 por ciento, en el hogar y escuela 29,17 por ciento. No existen limitaciones (infraestructura, materiales y personal) para ejecutar el programa. Se compararon los porcentajes de las metas del programa con los encontrados. Una cobertura de 11,93 por ciento para el 2000, 11,39 por ciento el 2001 y 7,77 por ciento el 2002 un promedio de 10,36 por ciento de cobertura, siendo la meta 30 por ciento de pesquisas citológicas adecuadas