Quistes de ovario en la urgencia: ¿un dilema terapéutico para el cirujano general? / Ovarian cysts in the urgency: a therapeutic dilemma for the general surgeon?

Ovarian cysts occur in women of all ages. In young women, the majority are benign, and the incidence of malignancy is maximal in the fifth to seventh decades. An ovaian cyst may present to the emergency surgeon as a pelvic or abdominal mass. it may also be discovered as an incidental finding on pelvic examination. An ultrasound scan yields valuable information about the nature of an ovaian cyst. The aim of this report was to present an experience with this pathology with laparoscopic management and with a revision of the literature

Towards the physical map of the Trypanosoma cruzi nuclear genome: construction of YAC and BAC libraries of the reference clone T. cruzi CL-Brener

Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantage of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2.770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease.

Frecuencia de anticuerpos sericos anti-vibrio parahaemolyticus en manejadores de alimentos / Frequency of serum antibodies to Vibrio parahaemolyticus in seafood manipulators

Se determino la frecuencia de anticuerpos anti Vibrio parahaemolyticus en diversas poblaciones de sujetos asintomaticos seleccionados basandose en la ocupacion que desempenaban. Se emplearon para tal efecto dos metodos de aglutinacion que difieren solamente la forma de preparar el antigeno el metodo del antigeno "vivo" y el antigeno "muerto". La especificidad de la prueba se demostro al descartar la reactividad de cruzada de Vibrio parahaemolyticus con las enterobacterias mas comunes. De 100 personas encargadas de expender alimentos al publico se encontro que 17 tenian anticuerpos sericos para el germen. Diez personas de esta poblacion tenian mayor contacto con productos derivados del mar, demostrandose anticuerpos en cinco de ellas; asociacion que es significativa desde el punto de vista estadistico. Finalmente, se encontro en la poblacion general una frecuencia de 16.5% de sujetos con anticuerposnti Vibrio parahaemolyticus.Estos datos indican que las infecciones por Vibrio parahaemolyticus en nuestro medio mas aun entre personas manejadoras de alimentos procedentes del mar