X-ray repair cross-complementing group 1 Arg399Gln genetic polymorphism and risk of hepatocellular carcinoma in an Iranian population

Background: The association between X-ray repair cross-complementing group 1 [XRCC1] Arg399Gln gene polymorphism and hepatocellular carcinoma [HCC] has been investigated in several populations. However, the findings are controversial. The aim of this study was to address the association between XRCC1 Arg399Gln polymorphism and HCC in an Iranian population

Methods: We have evaluated the association between XRCC1 Arg399Gln gene polymorphism and HCC in 151 Iranian individuals [50 patients with HCC and 101 healthy matched controls] using polymerase chain reaction-restriction fragment length polymorphisms [PCR-RFLP] method

Results: Significant association was found for the XRCC1 A allele and HCC [OR = 1.93, 95% CI [1.16 - 3.25], P = 0.0099]. Also, genotype analysis by SNPStats online software showed a significant association between XRCC1 gene polymorphisms and HCC under co-dominant, dominant, and recessive genetic models

Conclusion: Our study provides evidence that the XRCC1 Arg399Gln polymorphism may be associated with the risk of HCC development in Iranian population

Association of the epidermal growth factor gene +61A>G polymorphism with hepatocellular carcinoma in an Iranian population

Aim: The aim of this study was to address the association of the EGF gene +61A/G polymorphisms and HCC susceptibility in an Iranian population

Background: The association of epidermal growth factor [EGF] gene +61A/G polymorphism [rs4444903] and hepatocellular carcinoma [HCC] has been investigated in several populations. However, the findings are controversial

Methods: A total of 40 unrelated HCC patients and 106 healthy individuals were enrolled in this study. Genomic DNA of HCC patients was extracted from formalin-fixed, paraffin-embedded samples using CinnaPure DNA kit according to manufacturer's instructions. Genomic DNA of healthy individuals, also, was extracted from peripheral blood cells using the boiling method. The rs4444903 [A/G] polymorphism was genotyped using the polymerase chain reaction [PCR]-restriction fragment length polymorphism [RFLP] method

Results: Significant association was found for the EGF +61A allele and HCC risk [OR = 1.72, 95% CI [1.02 - 2.90], P value = 0.04]. Also, significant association was observed for the EGF +61A/G genotypes and HCC risk under codominant and dominant models by SNPStats software analysis

Conclusion: Our findings suggest that the EGF gene +61A/G polymorphism [rs4444903] might be a risk factor for susceptibility to HCC in Iranian population. However, further studies using more samples are needed