RESUMO
Background: Gastroenteritis due to Salmonella is common in human and considered as a global dilemma of public health. This study was done to determine cmlA/tetR, bla PSE-1, bla TEM and sip B in the Salmonella strains by Multiplex-PCR method and their antibiotic susceptibility profile
Materials and methods: In this cross-sectional study, 163 clinical samples were obtained from patients admitted to Hazrat-e Rasool General Hospital. The antibiotic susceptibility test was determined using the disk diffusion method agreeing with CLS1 guideline. Then, M-PCR was achieved for determination of these target genes by the specific oligonucleotides primers
Results: Of 163 collected samples, 48[29.4%] Salmonella spp., were obtained, which 25[52.1%] were S. enteritidis, 14[29.2%] S. typhimurium and 9[18.7%] S. infantis. Antibiotic resistance analysis showed that the highest resistance rate were related to tetracycline [n: 27, 56.2%] and then streptomycin and chloramphenicol [n: 15, 31.2%]. All isolates [n: 48, 100%] were susceptible to imipenem, amikacin, gentamycin and trimethoprim/sulfamethoxazole. The MPCR results revealed that 62.5% and 16.6% of Salmonella spp., isolates carried cml/tetR and sipB genes, respectively
Conclusion: According to our results, detection and genotyping of virulence genes and comparison with global ranging is a basic requirement in the control and prevention of salmonellosis in industrial purposes