RESUMO
The petal's hydro-alcoholic extract of Rosa damascene Mill. on ileum contractions of Wistar rats and its possible mechanism were investigated. Forty-eight male Wistar rats were divided into six groups. Ileum was placed adjacent to propranolol [1 microM], naloxone [1 microM] and L-NAME [100 microM] and also under the influence of different doses [2-8 mM] of calcium chloride. Cumulative extract of R. damascene Mill. [100, 500, and 1000 mg/L] decreased ileum contractions induced by KCl [60 mM] in a dose-dependent manner [P < 0.0001]. Propranolol and naloxone significantly decreased the inhibitory effect of the extract on contractions induced by KCl [P < 0.001], but L-NAME was ineffective. Furthermore, calcium led to the contraction of depolarized tissue through KCI and this contractile effect decreased significantly induced by the cumulative concentrations of the extract [P < 0.001]. The results indicate that R. damascene Mill. Dose-dependently [100, 500, and 1000 mg/L] decreases ileum movements of the rat probably through stimulating the beta-adrenergic and opioid receptors and voltage-dependent calcium channels, and it may be used to treat digestive disorders