RESUMO
Objective: To study the chemical constituents from Orobanche cernua in Orobanchaceae. Methods: The chemical constituents were separated and purified by macroporous resin, silica gel, Sephadex LH-20, and MCI column chromatographies. Their structures were determined by physicochemical properties and spectral data. Results: Twelve compounds were isolated from 70% ethanol extract of O. cernua. Among them, eight phenylpropanoid glycosides were identified as acteoside (1), campneoside II (2), crenatoside (3), campneoside I (4), isocrenatoside (5), isoacteoside (6), leucosceptoside A (7), and cistanoside F (8), three liganans were identified as (+)-syringaresinol-4'-O-β-D-glucopyranoside (9), (+)-pinoresinol-4'-O-β-D-glucopyranoside (10), and isoeucommin A (11), and one steroide was stigmasterol-3-O-β-D-glucoside (12). Conclusion: Compounds 7 and 12 are isolated from the family Orobanchaceae for the first time; Compounds 4, 7, and 9-12 are isolated from the plants of Orobanche L. firstly, and compounds 2, 4, and 6-12 are found from O. cernua for the first time.