PCAT4 drives breast cancer progression by up-regulating URGCP expression through sponge miR-508-5p / 国际外科学杂志

Objective:To investigate the driving effect of prostate cancer associated transcript 4 (PCAT4) on the up-regulation of upregulator of cell proliferation (URGCP) expression in breast cancer progression through sponging miR-508-5p.Methods:The microarray data of lncRNA and miRNA with differential expression in breast cancer tissue were analyzed by Cancer Genome Atlas. The expression of PCAT4 in breast cancer was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR). Cell proliferation was measured by MTT and colony formation, cell apoptosis was analyzed by TUNEL, and cell migration and invasion were analyzed by Transwell. The correlation between PCAT4 and miR-508-5p, and miR-508-5p and URGCP was analyzed by RNA pull-down and double luciferase assay. Tumor xenograft studies were performed to analyze the correlation between PCAT4/miR-508-5p/URGCP axis and breast cancer cell growth in vivo. Measurement data were expressed as mean ± standard deviation ( ± s). T-test was used for comparison between two groups, and one-way analysis of variance was used for comparison between multiple groups. The correlation between PCAT4 and URGCP and miR-508-5p expression was evaluated by Pearson correlation analysis. Results:The expression level of PCAT4 was up-regulated in breast cancer tissues and cells. Knockout of PCAT4 inhibited cell proliferation and metastasis and promoted cell apoptosis. miR-508-5p was the target of PCAT4 and was negatively correlated with PCAT4. Overexpression of miR-508-5p in breast cancer can inhibit cell growth, migration and invasion, and promote cell apoptosis. URGCP is the target of miR-508-5p and induces progression of breast cancer. Tumor xenograft studies showed that PCAT4 drives breast cancer progression by affecting miR-508-5p/URGCP.Conclusion:The expression of PCAT4 is up-regulated in breast cancer tissues and cells, and PCAT4 can act as a molecular sponge of miR-508-5p, and significantly promote breast cancer progression by activating URGCP protein expression.

Osteosarcopenic obesity syndrome / 中华老年医学杂志

At present, we have a very limited understanding of the etiology, risk factors and pathogenesis of osteosarcopenic obesity(OSO)syndrome, but it is necessary to predict its negative impact on the human body, especially in the elderly.Recent studies have found that advisable resistance training combined with nutritional supplement can effectively improve or delay the occurrence of OSO syndrome, and has beneficial effects in resisting the decline of body function.

Osteosarcopenic obesity syndrome / 中华老年医学杂志

At present,we have a very limited understanding of the etiology,risk factors and pathogenesis of osteosarcopenic obesity(OSO)syndrome,but it is necessary to predict its negative impact on the human body,especially in the elderly.Recent studies have found that advisable resistance training combined with nutritional supplement can effectively improve or delay the occurrence of OSO syndrome,and has beneficial effects in resisting the decline of body function.

The gut microbiome: a therapeutic target in ischemic stroke / 国际脑血管病杂志

Numerous studies have shown that gut microbiota can influence the progression and prognosis of various nervous system diseases by regulating the immune response, including ischemic stroke. Studies have demonstrated that gut microbiota plays an important role in the pathogenesis and progression of ischemic stroke, so it is possible to treat ischemic stroke by regulating gut microbiota. This article summarizes the relationship between gut microbiota and ischemic stroke, and targeting the gut microbiota is a newdirection in the treatment of ischemic stroke.

Early identification and prevention of stroke-associated pneumonia / 国际脑血管病杂志

Stroke-associated pneumonia (SAP) is a complication with the highest mortality rate in patients with stroke.It is very important to accurately predict and give early prophylactic treatment.This article reviews the risk factors and predictors of SAP,as well as early identification and prevention.

Application of nasolabial flaps in repair of defects after midfacial skin tumor removal / 中华医学美学美容杂志

Objective To investigate the methods and effects of nasolabial flaps for repair of defects after skin tumor removal on midface.Methods From Augest 2012 to March 2015,the different flaps from nasolabial fold were utilized to repair 22 skin defects due to surgical removal of skin tumors on midface.There were 12 flaps with subcutaneous pedicle and 10 flaps with skin pedicle in the group.The size of the defects ranged from0.5cm × 1.0cmto4.0cm × 5.0cm.Results After operation,all flaps survived with primary healing,cosmetic results were satisfactory with conceal scar and no facial organs distortion after 1 month to 2 years following-up.No tumor recurrence occurred.Conclusions Thenasolabial flaps with appropriate pedicles could obtain the satisfactory results for the reconstruction of medium-sized skin defects on midface,especially suitable for middle-aged and elderly patients.