RESUMO
Objective:To evaluate the diagnostic value of KaVo DIAGNOdent(DD)by comparing the diagnostic results of visual examination(VE)and stereomicroscope.Methods:The occlusal surface of 68 extracted teeth were examined by DD and VE,confirmed the examining points and marked,then cut these teeth along the markers.The results of stereomicroscope were classified into D0,D1,2,D3,4 according to the occlusal caries' depth,and recognized as gold standard.The difference of sensitivity,specificity between VE and DD,and their coherence were analyzed.Results:Sensitivity of DD,in D3,4 level of occlusal caries,was the highest.Compared to the results of stereomicroscope,the accuracy of DD and VE was the highest in D3,4 level.The coherence of DD and VE was low in all levels.Conclusion:The present cut-off point of DD affects the assessment of DD diagnostic result.Future study about key points of diagnosis of DD can widen its use in clinic.
RESUMO
Objective:To investigate the correlation between human herpesvirus-6(HHV-6) infection and pathogenesis of oral squamous cell carcinoma.Methods:Samples of saliva, peripheral blood mononuclear cells (PBMCs),tumor tissue and tumor adjacent tissue were collected from 82 patients with oral squamous cell carcinoma, 25 patients with oral precancer lesion and 40 healthy young adults(controls). Nested PCR assay was used to examine HHV-6 infection.Results:1)In control, precancer lesion and carcinoma groups, HHV-6 positive rates(%) were 45.0,56.0, 72.0 in saliva, 22.5,48.0,57.3 in PBMCs, and general rates(%) were 47.5,56.0 and 80.5 respectively(control vs carcinoma,P0.05).Conclusions:Saliva gland is the position of HHV-6 latency/proliferation and saliva is the predominant route of HHV-6 transmission; HHV-6 infection correlates to the pathogenesis of oral squamous cell carcinoma.